毛细管电泳法对乳及乳制品中乳源蛋白的测定  

Milk Proteins Determination in Milk and Milk Products by Capillary Electrophoresis

作　　者：田兰[1] 陈春丽[1] 马晓丽[1] 孟磊[1] 热娜.卡斯木[1] 

机构地区：[1]新疆医科大学药学院,新疆乌鲁木齐830011

出　　处：《食品研究与开发》2013年第4期81-84,共4页Food Research and Development

基　　金：新疆维吾尔自治区高校科研计划青年教师启动基金(XJMEDU2008S39)

摘　　要：采用毛细管电泳方法对乳及乳制品中乳源蛋白成分进行检测。选择聚乙烯醇涂层毛细管,采用柠檬酸缓冲体系,在紫外检测214 nm、分离电压20 kV条件下对乳及乳制品中的α-乳白蛋白(α-La)、β-乳球蛋白(β-Lg)、α-酪蛋白(α-CN)、β-酪蛋白(β-CN)和k-酪蛋白(k-CN)进行分离测定。五种蛋白线性良好,线性相关系数均大于0.997 8,各蛋白峰面积的相对标准偏差为1.76%～3.28%,加标回收率范围为88.1%～110.8%。应用该方法对液态奶、酸奶及奶粉中的乳源蛋白进行测定。本法准确、简便、易行,适于测定液态奶、酸奶、奶粉中蛋白质的检测。The contents of milk proteins were determined by capillary electrophoresis in milk and milk products. The proteins of α-La , β-Lg ,α-CN ,β-CN and k-CN were separated and analyzed by using polyvinyl alcohol coated capillary column and citric acid buffer solution with running voltage of 20 kV and UV detection at 214 nm. The results showed that ： Linearity of the calibration curves was rather good with correlation coefficients of above 0.997 8. The relative standard deviation were from 1.76 % to 3.28 %. Recovery from samples were from 88.1% to 110.8 %. This method is accurate ,simple ,rapid,and reliable. It is suitable for the determination of milk proteins in liquid milk , sour milk and milk powder.

关 键 词：毛细管电泳 牛奶 乳制品 蛋白 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程] O658.9[轻工技术与工程—食品科学与工程]