顺序注射-紫外可见分析系统用于酶法检测尿酸  

Sequential Injection UV/Vis Spectrometric Enzymatic Assay for the Determination of Uric Acid

作　　者：胡晶[1] 祝蕾[1] 王珍妮[1] 王佩瑶[1] 杨梅[1] 

机构地区：[1]辽宁师范大学化学化工学院,辽宁大连116029

出　　处：《广州化工》2013年第8期110-112,共3页GuangZhou Chemical Industry

基　　金：国家自然科学基金项目(No:51072074)

摘　　要：利用已构建的顺序注射-紫外可见分析系统,基于尿酸氧化酶催化尿酸和辣根过氧化物酶催化过氧化氢的酶偶联反应,建立了在线检测尿酸的新方法。在最优实验条件下,对方法的分析性能进行了评价。结果表明,所建立的方法线性范围为0.025～0.60 mmol/L,检出限为0.01 mmol/L,能够满足临床分析要求;且分析速度快、试剂试样消耗量小。因此本方法有望应用于人血清尿酸水平的监测。Based on the enzyme -coupled reaction of urate oxidase and horseradish peroxidase, the online detection method for uric acid was developed by using the established sequential injection UV/Vis spectrometric system. Under the optimal experimental conditions, the analytical performance of this method was evaluated. This procedure not only provid- ed a linear range of 0. 025 -0. 60 mmol/L and detection limit of 0.01 mmol/L, which met the needs of the clinical analysis of uric acid, but also minimized sample and reagent consumption.

关 键 词：尿酸 酶法 顺序注射-紫外可见分析系统 

分 类 号：O657.32[理学—分析化学]