远志对阿尔茨海默病大鼠海马神经元Tau蛋白磷酸化的影响  

Effect of Polygala on Tau Protein Phosphorylation of Hippocampal Neurons of Alzheimer Disease Rat Models

作　　者：穆俊霞[1] 郭锋[2] 崔丽霞[3] 李新毅[4] 

机构地区：[1]山西中医学院,山西太原030024 [2]吕梁市人民医院,山西离石033000 [3]长治医学院附属和平医院,山西长治046000 [4]山西医科大学附属大医院,山西太原030032

出　　处：《山西中医学院学报》2013年第1期22-24,共3页Journal of Shanxi College of Traditional Chinese Medicine

基　　金：山西省科技攻关项目(20080312018-5);山西省卫生厅科技攻关项目(200751)

摘　　要：目的:研究远志对阿尔茨海默病(Alzheimer′s disease,AD)大鼠模型海马神经元Tau蛋白磷酸化的影响。方法:将32只雄性健康Wistar大鼠随机分为对照组,模型组,远志1组,远志2组。用脑立体定位技术给大鼠海马注射Aβ1-40与apoE4的混悬液构建AD模型。将大鼠断头取脑,分离海马,采用免疫组化染色法检测大鼠海马神经元Tau蛋白磷酸化的情况。结果:①与对照组比较,模型组、远志1组、2组磷酸化Tau蛋白的平均灰度值明显降低(P<0.01)。②与模型组比较,远志1组、2组磷酸化Tau蛋白的平均灰度值明显升高(P<0.01)。远志2组与远志1组比较,磷酸化Tau蛋白的平均灰度值明显升高(P<0.01)。结论:远志对可溶性Aβ1-40与apoE4联合注射所致AD大鼠模型海马CA1区神经细胞内Tau蛋白过度磷酸化有明显的改善作用。Objective：To observe the effect of polygala on Tau protein phosphorylation of hippocampal neurons of Alzheimer′s disease（AD） rat models.Methods：32 male rats were randomly divided into control group,model group,polygala 1 group and 2 group.AD models were established by injecting bilaterally mixed liquor of Aβ1-40 and apoE4 into the hippocampus CAl regions under the control of stereotaxic apparatus.Brains of rats were removed and hippocampus neuron were isolated,then Tau protein phosphorylation was detected by immunohisochemistry staining.Results：Compared with control group,average gray value of Tau protein phosphorylation was significantly decreased in model group,polygala 1 group and 2 group（P0.01）.Compared with model group,average gray value of Tau protein phosphorylation was significantly increased in polygala 1 group and 2 group（P0.01）.Average gray value of Tau protein phosphorylation in polygala 2 group were higher than that in polygala 1 group（P0.01）.Conclusion：Polygala has apparent propective effect on Tau protein hyperphosphorylation in nerve cells of hippocampus CAl regions of AD rat models induced by injecting mixed liquor of Aβ1-40 and apoE4.

关 键 词：远志 阿尔茨海默病 TAU蛋白 

分 类 号：R749.1[医药卫生—神经病学与精神病学]