硫酸镁对心肺复苏后大鼠大脑皮质水肿和水通道蛋白4基因表达的影响  

Influence of magnesium suffate to the cortical edema and genic expression of aquaporin - 4 of cerebral cortex on the rats after cardiopulmonary resuscitation

作　　者：陆件[1] 张艳[1] 刘励军[1] 

机构地区：[1]苏州大学附属第二医院急诊与重症医学科,江苏苏州215000

出　　处：《中国急救医学》2013年第4期347-350,共4页Chinese Journal of Critical Care Medicine

摘　　要：目的探讨硫酸镁（MgSO4）对心肺复苏（CPR）后大鼠大脑皮质水肿和水通道蛋白g（AQP4）基因表达的影响。方法68只健康成年雄性SD大鼠随机分为三组：假手术组、CPR模型组、MsSO4治疗组。每组再分四个亚组：即自主循环恢复（ROSC）后3、6、12、24h组，除了假手术组各时相点为5只大鼠外，另两组大鼠各时相点均为6只。MgSO4治疗组在ROSC后立即行MgSO4干预。应用干湿质量法测定脑皮质含水量（cerebral cortex water content，CCWC），逆转录一聚合酶链反应（RT-PCR）技术测定脑皮质AQP4 mRNA的表达水平。结果MgSO4治疗组ROSC后3、6、12、24hCCWC和AQP4 mRNA表达水平与相同时点CPR模型组比较均明显降低（P〈0．05）。结论MgSO4可减轻CPR后脑水肿，其机制可能是降低了AOP4的表达。Objective To explore the effects of magnesium sulfate （MgSO4）on cerebral edema and expression of aquaporin- 4（AQP4） in cerebral cortex after cardiopulmonary resuscitation（CPR） in rats. Methods 68 healthy adult SD rats, were randomly divided into 3 groups ： sham （ n = 20）, CPR model group, MgSO4 treatment group. Each group then divided into 4 subgroups, that is 3 h, 6 h, 12 h, 24 h subgroup after Restoration of Spontaneous Circulation （ ROSC）, each subgroup n = 6, except for sham. The rats of MgSO4 group were treated by MgSO4 at once after ROSC. The cerebral cortex water content（CCWC） was determined by wet -to -dry weight ratio, the level of AQP4 mRNA expression was determined by Reverse Transcription - Polymerase Chain Reaction （ RT - PCR）. Results the CCWC and expression level of AQP4 mRNA were Significantly lower in MgSO4 treatment group than the CPR model group （ P 〈 0. 05 ）. Conclusion MgSO4 can reduce cerebral edema after CPR, and its mechanism perhaps down - regulates the expression of AQP4 in brain tissue.

关 键 词：硫酸镁(MgSO4) 心肺复苏(CPR) 水通道蛋白4(AQP4) 脑水肿 

分 类 号：R-33[医药卫生]