不同电子供体下三氯苯酚的还原脱氯机制研究  

Mechanism of Reductive Dechlorination of Trichlorophenol with Different Electron Donors

作　　者：万金泉[1] 胡梦蝶[1] 马邕文[2] 黄明智[1] 

机构地区：[1]华南理工大学环境科学与工程学院,广州510006 [2]华南理工大学制浆造纸工程国家重点实验室,广州510640

出　　处：《环境科学》2013年第5期1808-1814,共7页Environmental Science

基　　金：2011年广东省高层次人才基金项目(2011);广东省战略性新兴产业核心技术攻关项目(2012A032300015)

摘　　要：研究了葡萄糖、乳酸钠、丙酮酸钠、乙酸钠这4种电子供体条件下,2,4,6-三氯苯酚(2,4,6-trichlorophenol,2,4,6-TCP)的降解效果及其还原脱氯途径.结果表明,与葡萄糖的作用相比,乳酸钠、丙酮酸钠、乙酸钠均可有效提高2,4,6-TCP的脱氯效果,其中乳酸钠能作为一类缓释氢物质持续供给2,4,6-TCP还原脱氯所需电子.外加电子供体可提高微生物体内基质代谢脱氢酶活性,反应240 h后,4种电子供体体系中脱氢酶活性增长依次为21.49%、25.78%、136.85%和139.3%.2,4,6-TCP还原脱氯的主要产物包括2,4-二氯苯酚(2,4-dichlorophenol,2,4-DCP)、4-氯苯酚(4-chlorophenol,4-CP)和苯酚,其中乙酸钠作为电子供体时,4-CP为其主要降解产物,转化率达到22%以上.A test was conducted to examine the degradation effect and reductive dechlorination pathway of 2,4,6-trichlorophenol（2,4,6-TCP） in the presence of different electron donors,such as glucose,sodium lactate,sodium pyruvate and sodium acetate.The results showed that,compared with the effect of glucose,sodium lactate,sodium pyruvate and sodium acetate enhanced the dechlorination of 2,4,6-TCP effectively,among which sodium lactate could serve as a kind of hydrogen release compound,and the electrons required for reductive dechlorination were released in a sustained way.Substrate metabolism dehydrogenase activity was improved by the external electron donor;after reaction for 240 h,the activity of dehydrogenase was increased in the four electron donor systems,by 21.49%,25.78%,136.85% and 139.3%,respectively.The main reductive dechlorination products of 2,4,6-TCP included 2,4-dichlorophenol（2,4-DCP）,4-chlorophenol（4-CP） and phenol;when sodium acetate was used as the electron donor,4-CP was the main degradation product,and the transformation ratio from 2,4,6-TCP to 4-CP was more than 22%.

关 键 词：2 4 6-三氯苯酚 还原脱氯 电子供体 电子传递体系活性 脱氢酶 

分 类 号：X172[环境科学与工程—环境科学]