急性脑梗死患者血小板内钙和TXB_2含量与聚集关系的探讨  

Relationships cytosolic calcium levels, thromboxane B2 levels of intraplatelet and platelet aggrepation induced by ADP in acute cerebral infarction

作　　者：余仲苟[1] 马中富[2] 黄帆[2] 刘秀琴 梁秀龄[2] 

机构地区：[1]中山医科大学附属第一医院急诊科,广州510080 [2]中山医科大学附属第一医院神经科,广州510080 [3]中山医科大学附属第一医院核医学教研室,广州510080

出　　处：《中国急救医学》2000年第4期210-211,共2页Chinese Journal of Critical Care Medicine

摘　　要：目的探讨急性脑梗死急性期患者血小板内游离钙和ＴＸＢ２含量之间的相互关系及意义。方法　用Ｆｕｒａ－２／ＡＭ荧光技术测定了４６例急性脑梗死患者急性期及２５例正常对照组血小板内钙含量，用放射免疫分析法测定其血小板内血栓素Ｂ２（ＴＸＢ２）含量，用比浊法测定其血小板聚集。结果患者血小板内游离Ｃａ２＋和ＴＸＢ２含量均分别明显高于正常对照组（１４７．２６±１０．８５ ｖｓ １１９．３０±１０．９４ ｎｍｏｌ／Ｌ和３．３８±０．７８ｖｓ　４．８８±０．９７ｎｇ／１０９血小板，Ｐ均＜０．０１）；在患者中，低（０．５μｍｏｌ／Ｌ）和高（５μｍｏｌ／Ｌ）浓度二磷酸腺苷（ＡＤＰ）诱导的血小板聚集均明显高于正常对照组（０．４４±０．１９ｖｓ　０．２６±０．１４和０．７３±０．１３ｖｓ０．３９±０．１９，Ｐ均＜０．０１）；在患者中血小板内游离Ｃａ２＋含量和ＴＸＢ２含量均分别与５μｍｏｌ／Ｌ二磷酸腺苷诱导的血小板呈明显的正相关性（ｒ分别为０．７８６和０．５９３，Ｐ均＜０．０１），而血小板内Ｃａ２＋与ＴＸＢ２成明显的正相关性（ｒ为０．６５２，Ｐ＜０．０１）。结论 急性脑梗死患者在急性期血小板功能处于活跃状态，可能与其内 Ｃａ２＋含量增加。Objective To investigate the relationships and the significantly among cytosolic calcium levels thromboxane B2 levels of intraplatelet and platelet aggregation induced by ADP in the patients with acute cerebral infarction (ACI). Methods Cytosolic calcium levels were measured in Fura - 2/AM loaded platelet in 46 patients with ACI and in 25 normal controls, platelet thromboxane B2 levels were investigated by radioimmunoassy (RIA)and platelet aggrega- tion induced by ADP were measured with lumi - aggregometer M450. Results The intraplatelet levels of calcium and thromboxane B2 in the patients with ACI were significantly higher than those in the nounal controls, respectively (147.26±10.85 vs 119.30±10.94 nmol/L, and 3.38±0.78 vs 4.88±0.97 ng/109 platelet, respectively, all P < 0.01 ), but platelet induced by 0. 5 μmol/L ADP and 5 μmol/L ADP in the patients were significantly lower than those in normal con- trols, respetively(0.44±0. 19 vs 0.26 ±0. 14, and 0.73± 0. 13 vs 0. 39 ± 0. 19, respetively, all P < 0.01 ). In the patients, moreover, the intraplatelet calcium levels and intraplatelet thromboxane B2 levels were positively correlated with the aplatelet aggregation induced by 5μmol/LADP (r were 0.786 and 0. 593, respec- tively, all P<0.01 ). And there was a positive relationship between intraplatelet calcium levels and intraplatelet thromboxane B2(r was 0.652 P < 0.01 ).Con- clusions Platelet was activative during the acute phase in the patients with ACI. which was possible related to the increase of cytosolic calcium levels and throm- boxane B2 levels in intraplatelet. Their interaction activated platelet in ACI .These data suggested that using antiplatelet durgs might play an important role in the treatment of ACI.

关 键 词：血小板 血栓素B2 血小板聚集 脑梗塞 

分 类 号：R743.33[医药卫生—神经病学与精神病学]