p62参与人恶性黑色素瘤细胞NF-κB活性调控  

The Role of p62 Involves in the Regulation of NF-κB Activity in Human Malignant Melanoma cells

作　　者：李亚平[1] 饶艳伟[2] 谷爽[3] 李晓宁[4] 周彤[4] 马丽伟[4] 杨晓春[4] 苏静[4] 

机构地区：[1]吉林省人民医院皮肤科,吉林长春130021 [2]吉林省人民医院ICU科,吉林长春130021 [3]吉林省人民医院胸外科,吉林长春130021 [4]吉林大学白求恩医学院病理生理学系

出　　处：《中国实验诊断学》2013年第4期622-625,共4页Chinese Journal of Laboratory Diagnosis

基　　金：国家自然科学基金面上项目(81141099;81202552;81272876);吉林省科技厅资助项目(200705373)

摘　　要：目的探讨多功能蛋白p62参与调控人恶性黑色素瘤细胞NF-κB活性的机制。方法选取人恶性黑色素瘤标本20例,免疫组化方法检测p50、p65、p62表达。人黑色素瘤A375细胞,转染p62-siRNA特异性表达载体。MTT法检测细胞生存率,western blot检测p62蛋白表达,NF-κB荧光素酶报告基因分析检测NF-κB活性。结果 Spearman等级相关性分析p50与p65、p65与p62表达呈正相关均有统计学意义(P<0.05)。与对照组相比,A375细胞转染p62-siRNA特异性表达载体24h、48h和72h,p62蛋白表达、细胞生存率和NF-κB活性均明显降低(P<0.05)。结论人恶性黑色素瘤中p62表达与NF-κB高表达呈正相关,降低p62表达能通过抑制NF-κB活性降低A375细胞存活率,表明p62参与NF-κB活性调控。Objective To investigate p62 involves in regulation of NF-κB activity in human malignant melanoma cells. Methods The expression of p50 ,p65,p62 in twenty cases of human malignant melanoma specimens were detected by immunohistochemical assay. Human melanoma A375 cells were transfected with p62-siRNA-specific expression vec- tor. Cell viability was detected by MTT assay. The expression of p62 was detected by western blot. The NF-κB activi- ty was detected by NF-κB luciferase reporter gene assay. Results Spearman rank correlation analysis of p50 and of p65, p65 and p6-2 were statistically significant positive correlation （P〈0.05）. Compared with the control group,A375 cells transfected with p62-siRNA-specific expression vectors 24 h,48 h and 72 h, expression of p62, cell viability and NF-κB activity were significantly decreased （P〈0.05）. Conclusion The expression of p62 was positively correlated with the high expression of NF-κB. Inhibiting NF-κB activity by p62 suppression reduces the A375 cell survival,indi- cates that p62 involved in the regulation of NF-κB activity.

关 键 词：恶性黑色素瘤 核因子ΚB 多功能蛋白p62 

分 类 号：R739.5[医药卫生—肿瘤]