慢性乙醇中毒脑β-淀粉样前体蛋白与外伤性蛛网膜下腔出血死亡的关系  

Relation between Expression of Cerebral β-APP in the Chronic Alcoholism Rats and Death Caused by TSAH

作　　者：魏来[1,2] 雷怀成[1] 于晓军[2] 赖小平[3] 钱红[4] 徐小虎[2] 朱方成[1] 

机构地区：[1]湖北医药学院法医学教研室,湖北十堰442000 [2]汕头大学医学院法医学教研室,广东汕头515041 [3]广东医学院法医学教研室,广东东莞523808 [4]南华大学附属南华医院病理科,湖南衡阳421002

出　　处：《法医学杂志》2013年第2期91-95,共5页Journal of Forensic Medicine

基　　金：国家自然科学基金资助项目(30772458)

摘　　要：目的观察大鼠慢性乙醇中毒下脑组织轻微伤后β-淀粉样前体蛋白(β-amyloid precursor protein,β-APP)的表达,探讨乙醇中毒与外伤性蛛网膜下腔出血(traumatic subarachnoid haemorrhage,TSAH)死亡的相关性。方法雄性SD大鼠60只,随机分为灌水组、灌水打击组、灌酒组、灌酒打击组。灌酒组以食用酒连续灌胃4周,打击组予脑震荡打击,各组大鼠脑组织行HE、免疫组织化学染色,组织病理图像系统量化分析检测结果。结果灌水组脑无异常;灌水打击组全脑神经元轻度水样变性;灌酒组脑表面可见血管纹理,神经纤维排列稍疏松、间隙增大;灌酒打击组脑表面弥漫性淤血,脑干周围多见厚层斑片状TSAH,轴索不规则断裂。灌酒组额叶、海马、小脑、脑干β-APP IOD值高于灌水打击组及灌酒打击组。结论慢性乙醇中毒脑组织的耐受性明显下降,轻微损伤情况下即可引起致死性TSAH和神经病理学变化。Objective By observing the cerebral β-amyloid precursor protein （β-APP） expression in the chron- ic alcoholism rats with slight cerebral injury, to discuss the correlation of chronic alcoholism and death caused by traumatic subarachnoid haemorrhage （TSAH）. Methods Sixty male SD rats were randomly di- vided into watering group, watering group with strike, alcoholism group and alcoholism group with strike. Among them, the alcohol was used for continuous 4 weeks in alcoholism groups and the concus- sion was made in groups with strike. In each group, HE staining and immunohistochemical staining of the cerebral tissues were done and the results were analyzed by the histopathologic image system. Results In watering group, there was no abnormal. In watering group with strike, mild neuronic congestion was found. In alcoholism group, vascular texture on cerebral surface was found. And the neurons arranged in disorder with dilated intercellular space. In alcoholism group with strike, diffuse congestion on cerebral surface was found. And there was TSAH with thick-layer patches around brainstem following irregular axonotmesis. The quantity of β-APP IOD in alcoholism group was significantly higher in the frontal lobe, hippocampus, cerebellum, brainstem than those in watering group with strike and alcoholism group with strike. Conclusion The cerebral tissues with chronic alcoholism, due to the decreasing tolerance, could cause fatal TSAH and pathological changes in cerebral tissues of rats under slight cerebral injury.

关 键 词：法医病理学 中毒 乙醇 淀粉样前体蛋白分泌酶类 蛛网膜下腔出血 创伤性 大鼠 

分 类 号：R743.35[医药卫生—神经病学与精神病学]