巴西海参Ludwigothurea grisea的体壁多糖类型、性质及活性研究  

Polysaccharide Types,Physicochemical Characteristics and Anticoagulant Activities of Polysaccharides from the Sea Cucumber Ludwigothurea Grisea

作　　者：罗兰[1] 赵金华[2] 向静颖[3] 李姿[2] 吴明一[2] 王盛民[1] 

机构地区：[1]西南交通大学,四川成都610031 [2]中国科学院昆明植物研究所,云南昆明650201 [3]云南中医学院,云南昆明650500

出　　处：《云南中医学院学报》2013年第2期14-19,共6页Journal of Yunnan University of Traditional Chinese Medicine

基　　金：国家自然科学基金青年科学基金项目(NO:81102372);云南省高端人才引进项目(NO:2010CI116);云南省应用基础研究计划--应用基础研究面上项目(NO:2012FB177)

摘　　要：目的研究巴西海参Ludwigothurea grisea所含多糖种类、理化性质及其活性。方法提取分离海参多糖,研究其理化性质、波谱特征和抗凝活性。结果 L.grisea体壁中得到3种多糖,化学结构及理化性质存在显著性差异,LG-1为硫酸酯取代的岩藻聚糖;LG-2由α-葡萄糖组成,为中性葡聚糖;LG-3由岩藻糖、葡萄糖醛酸和乙酰氨基半乳糖组成,为岩藻糖化糖胺聚糖。LG-3抗凝活性强于LG-1,LG-2无抗凝活性。结论此海参中至少含有3种不同类型的多糖,中性葡聚糖首次在海参中发现。它们的抗凝活性可能与单糖组成及硫酸酯基含量相关。Objective This work is to study the types of polysaccharides, physicochemical characteristics and biological activities of the sea cucumber Ludwigothurea griseca Methods The polysaccharides were isolated from the body wall of sea cucumber L. grisea. Their multiple physicochemical properties, spectral characteristics and anticoagulant activities were then examined. Results The three types of polysaccharides were found in the sea cucumber L. grisea, and their chemical structures and physicochem ical properties are significantly different each other. In addition, fraction LG-1 is a fucan with sulfate ester, the polysac charide fraction LG-2 is a neutral glucan which contains glucose as the only sugar, and fraction LG-3 iscomposed of glucuronic acid （GlcA）, N-acetylgalactosamine （GalNAc） and fucose, named fucosylated glycosaminoglycan （FGAG）. The LG-3 has stronger anticoagulant activities than LG-1, and the LG-2 has no activity. Conclusion The body wall of the sea cucumber L. grisea contains at least three different types of polysaccharides, among which the neutral a-glucan was firstly obtained from sea cucumber. The anticoagulant activities of the different types of polvsaccharides may relate to monosaccharide composition and sulfate content.

关 键 词：海参 多糖 理化性质 抗凝活性 

分 类 号：R284.1[医药卫生—中药学]