磷酸-丙酮预处理对水稻秸秆酶解糖化的影响  

Effects of phosphoric acid-acetone pretreatment on enzymatic saccharification of rice straw

作　　者：曾青兰[1] 李晓宏[1] 

机构地区：[1]咸宁职业技术学院生物工程系,咸宁437100

出　　处：《华中农业大学学报》2013年第3期77-81,共5页Journal of Huazhong Agricultural University

基　　金：湖北省自然科学基金项目(2011CDC092);湖北省教育厅科学技术研究项目(B20129303);咸宁职业技术学院院级重点课题基金(2012yja002)

摘　　要：为提高水稻秸秆木质纤维素的能源化利用效率,采用磷酸-丙酮对水稻秸秆进行预处理,研究预处理水稻秸秆粒径、固液比、温度、时间对纤维素酶水解糖化率的影响,并用扫描电镜分析预处理前后水稻秸秆结构的变化。结果表明,水稻秸秆预处理的适宜条件为:粒径≤380μm、固液比1∶8、温度80℃、处理时间为1.5 h,在此条件下预处理的水稻秸秆纤维素酶水解糖化率从未处理水稻秸秆的18.6%提高到预处理后的65.4%。SEM分析表明,经磷酸-丙酮预处理,水稻秸秆晶状结构遭到破坏,并崩解为碎片,从而使后续的纤维素酶水解糖化率显著提高。To improve lignocellulose energy-oriented use efficiency of the rice straw,rice straw was pretreated with phosphoric acid-acetone. The effects of particle size,materials to liquid ratio,temperature and time on enzymatic saccharification ratio of rice straw were studied. The construction of the rice straw was scanned before and after the pretreatment by SEM. The results showed that when the particle size of rice straw was 4380 /zm, the materials to liquid ratio was 1 ~ 8, the pretreated temperature was 80 ~C and the pretreated time was 1. 5 h, the enzymatic saccharification ratio of rice straw increased from 18. 6% to 65.4%. The crystalline structure of the rice straw was destroyed to pieces by SEM,providing good working conditions for the subsequent enzymatic saccharification. The research could provide an effective pretreated way of straw under mildness conditions.

关 键 词：磷酸 丙酮 水稻秸秆 纤维素 糖化 预处理 

分 类 号：S216.3[农业科学—农业机械化工程] X712[农业科学—农业工程]