生物活性材料支架诱导成年大鼠神经再生修复脑损伤的研究  

Repair of Injured Adult Rat Brain by Inducing Neural Regeneration with Bioactive Material Scaffolds

作　　者：陈兰[1] 孙敏[1] 张皑峰[2] 杨朝阳[1] 李晓光[1] 

机构地区：[1]首都医科大学神经生物学系,北京市100069 [2]北京友谊医院口腔科,北京市100050

出　　处：《中国康复理论与实践》2013年第4期318-323,共6页Chinese Journal of Rehabilitation Theory and Practice

基　　金：北京市科委重点项目(No.D09080104660000);国家自然基金重点项目(No.31130022);国家科技支撑计划(No.2012BAI17B04);国家"863"计划(No.2012AA020506)

摘　　要：目的应用生物活性材料支架诱导神经再生修复成年大鼠创伤性脑损伤。方法 52只成年雄性Wistar大鼠分为假手术组、单纯损伤组、单纯壳聚糖组和生物活性材料支架组,分别于术后3 d、7 d、2周、4周、2个月和3个月通过组织化学、免疫组织化学和神经示踪方法检测神经再生和损伤区新生神经元的来源。结果应用生物活性材料支架后,海马齿状回(DG)和室管膜下区(SVZ)中BrdU+/Nestin+和BrdU+/Dcx+细胞较单纯壳聚糖组和单纯损伤组明显增加。损伤区内BrdU+/Nestin+、BrdU+/Dcx+及BrdU+/MAP2+神经细胞较单纯壳聚糖组和单纯损伤组明显增加。DiI标记SVZ细胞后,损伤区中见DiI+/NeuN+神经元。结论生物活性材料支架激活DG和SVZ的神经干细胞,SVZ中的神经干细胞迁移到损伤区,并分化为成熟神经元。Abstract： Objective To repair the brain of adult rats with traumatic brain injury by inducing neural regeneration with biological scaf- folds. Methods 52 adult male Wistar rats were divided into control group, lesion group, blank chitosan carriers group and bioactive material scaffolds group. The neural regeneration and the origin of the newborn neurons in the injury area were detected through histochemistry, im- mumochemistry and neural tracing on the 3rd day, 7th day, 2rid week, 4th week, 2nd month and 3rd month after operation. Results After ap- plication of bioactive material scaffolds, the BrdU＋/Nestin＋ and BrdU＋/Dcx＋ cells in gyrus dentatus （DG） of hippocampus and subventricular zone （SVZ） were significantly more than those of the other groups. The BrdU＋/Nestin＋, BrdU＋/Dcx＋ and BrdU＋/MAP2＋ neural cells in injury area of the bioactive material scaffolds group were significantly more than those of the other groups. After application of DiI to label the SVZ cells, DiI＋/NeuN＋ neurons were observed in the injury areas. Conclusion Bioactive material scaffolds can activate the neural stem cells in SVZ and DG. The neural stem cells in SVZ can migrate to the injury area and then differentiate into mature neurons.

关 键 词：脑损伤 生物活性材料支架 神经干细胞 神经再生 迁移 分化 大鼠 

分 类 号：R745[医药卫生—神经病学与精神病学]