线粒体型天门冬氨酸氨基转移酶同工酶对肝脏疾病诊断价值的探讨  

The explore of the diagnosis value of mAST in patients with liver diseases

作　　者：杜宗孝[1] 李富荣[1] 朴文花[1] 

机构地区：[1]宁夏自治区人民医院临床医学检验诊断中心,宁夏银川750021

出　　处：《宁夏医学杂志》2013年第4期303-304,共2页Ningxia Medical Journal

基　　金：宁夏自治区科技攻关资助项目(2009232)

摘　　要：目的探讨线粒体型天门冬氨酸氨基转移酶同工酶(m-AST)及m-AST与天门冬氨酸氨基转移酶(AST)对肝脏疾病的诊断价值。方法采用免疫抑制法测定192例肝病患者的血清m-AST,并计算m-AST/AST。结果对照组86例m-AST活力为(8.8±1.4)U/L,肝病患者血清m-AST水平显著高于对照组(P<0.05);入院2周后急性肝炎m-AST及m-AST/AST比值下降明显,而慢性肝炎、肝硬化、肝癌组下降不明显(P>0.05);ROC曲线下对慢性肝炎组诊断价值最大。结论血清m-AST活力测定,有助于评估肝细胞坏死程度,判断疾病的发展,鉴别急、慢性肝脏疾病。Objective To investigate the clinical significances of serum mAST activities and mAST/AST ratio in patients with liver diseases. Methods The activities of the mAST was detected in 192 patients serum by using immunoinhibition assays. The mAST/ AST ratio was calculated. Results The activities of the mAST in 86 healthy controls serum were （8.8 ± 1.4） U/L. The activities of the mAST in patients with liver diseases was sharp higher than normal control group （P 〈 0.01 ） ; The m-AST activities and m -AST/AST ratio in acute hepatitis was significantly decreased after two weeks of treatment, while there was no significant decline in chronic hepati- tis, hepatitis cirrhosis and hepatocellular carcinoma （P 〉 0.05）. AUROC curve of mAST was valuable in the chronic hepatitis diagno-sis. Conclusion Dynamical monitor of the changes in the serum mAST activities may help to assess the extent which the substantial liver cells are damaged, the development of the disease, as well as the discrimination and diagnosis of acute and chronic hepatitis.

关 键 词：同工酶 线粒体型 天门冬氨酸氨基转移酶 肝脏疾病 

分 类 号：R575[医药卫生—消化系统]