茯砖茶对小鼠肠道免疫功能调节作用的研究  

Effects of Fuzhuan Brick Tea on the Adjustment of Intestinal Immune Function in Mice

作　　者：吴香兰[1,2] 刘仲华[1,2] 曹丹[1,2] 金莉莎[1,2] 林勇[1,2] 

机构地区：[1]国家植物功能成分利用工程技术研究中心,湖南长沙410128 [2]湖南农业大学茶学教育部重点实验室,湖南长沙410128

出　　处：《茶叶科学》2013年第2期125-130,共6页Journal of Tea Science

基　　金：湖南省重大科技专项"茯茶产业化关键技术研究与示范"(2008FJ1005);国家自然科学基金项目(31100502)

摘　　要：探讨了茯砖茶水提物对小鼠肠道免疫功能的调整作用。试验以注射用氨苄青霉素灌胃建立小鼠肠道菌群失调及免疫功能紊乱模型。昆明小鼠随机分6组,正常对照组、自然恢复组、模型组和茯砖茶高、中、低剂量组,分别进行肠道菌群检测、小肠黏液sIgA的测定、血清中IL-2的测定、血清白蛋白和总蛋白含量检测以及回肠切片病理检测。结果表明,注射用氨苄青霉素灌胃5 d小鼠,肠道菌群失调,小肠黏液sIgA、血清中IL-2、血清白蛋白和总蛋白均较正常组降低,回肠组织切片明显病变。分别灌服茯砖茶水提物后,肠道菌群得以调整,小肠黏液sIgA、血清中IL-2、血清白蛋白和总蛋白均有所升高,且与自然恢复组比具有显著性差异。各治疗组之间比较,高剂量组疗效优于低剂量组。说明茯砖茶水提物能修复受损黏膜,有调节肠道免疫功能的作用。The aim of this study was to investigate the effects of FU brick tea water extracts on the adjustment of intestinal immune function of mice. Lavage with the Ampicillin to establish mice intestinal dysbacteriosis and immune dysfunction model. KM mice were random divided into six groups： normal control, natural recovery group, model group, three dose group of brick tea. The intestinal flora detection, the small intestinal mucous slgA measurement, the measurement of IL-2 in serum, Serum albumin and total protein content detection and Ileum section pathological detection. The results showed that lavage with the Ampicillin 5 d, alteration of intestinal flora, the small intestinal mucous slgA, the IL-2 in serum, serum albumin and total protein content were declined significantly compared with the normal saline group, in addition, the Ileum tissue slice showed obvious lesions. Compared with the natural recovery group, the small intestinal mucous slgA, the IL-2 in serum, serum albumin and total protein content were increased significantly after the treatment with the brick tea water extracts. In the comparison between the treatment groups, the effect of high doses group were much better than the low doses group of treatment. It was concluded that the FU brick tea extracts can repair the damaged mucous membrane, showed the adjustment of the intestinal immune functions.

关 键 词：茯砖茶 肠道免疫 SIGA IL-2 肠道菌群 

分 类 号：R285.5[医药卫生—中药学]