甘肃黄芩中提取物黄芩苷元体外抑制假丝酵母菌研究  

Inhibitory effects in vitro of baicalein in Radix Scutellariae of Gansu on candida

作　　者：张美玲[1] 魏莲花[2] 王宏伟[1] 张兆芳[3] 田洹[1] 

机构地区：[1]甘肃中医学院附属医院检验科,甘肃兰州730020 [2]甘肃省临床检验中心,甘肃兰州730000 [3]甘肃中医学院附属医院制剂室,甘肃兰州730020

出　　处：《国际检验医学杂志》2013年第7期780-781,共2页International Journal of Laboratory Medicine

基　　金：甘肃省科技攻关计划项目(2GS064-A43-020-28)

摘　　要：目的找出甘肃黄芩中提取物黄芩苷元体外抑制假丝酵母菌的抗菌谱。方法将甘肃黄芩先制成黄芩苷,再用强酸加热水解,提取其中有效活性成分黄芩苷元,用甲醇溶解,制成黄芩苷元制剂。收集临床分离深部感染假丝酵母菌134株,传代2～3次保持其活力,制成0.5麦氏单位(1×106～5×106CFU/mL)菌悬液,将菌悬液接种于沙保弱药物平板上,35℃培养24～48h,观察被检菌生长情况,找出药物最低抑菌浓度(MIC)。结果黄芩苷元MIC试验确定,MIC分别为:白色假丝酵母菌0.956μg/mL,光滑假丝酵母菌1.047μg/mL,热带假丝酵母菌和葡萄牙假丝酵母菌1.138μg/mL,克柔假丝酵母菌和丛生丝孢假丝酵母菌1.411μg/mL。结论黄芩苷元在体外对白色假丝酵母菌抑制效果最好,光滑假丝酵母菌次之,热带假丝酵母菌和葡萄牙假丝酵母菌较差,克柔假丝酵母菌和丛生丝孢假丝酵母菌最差。Objective To seek the antibacterial spectrum of baicalein in Radix Scutellariae of Gansu on inhibitory effects in vitro for Candida. Methods The baicalin in Radix Scutellariae of Gansu was prepared,and used strong acid to conduct by heating hydrol- ysis,the baicalein that is effective active component of Radix Scutellariae was extracted,and it was dissolved by methanol to make baicalein reagent. 134 strains Candida from deep fungal infection focus in clinic were isolated and collected, and were cultured in vitro 2 to 3 times to maintain vitality, and were made of 0.5 wheat＇s unit（1 ×106 to 5×106） of bacterial suspension. The bacterial suspension was spread on Sabouraud drug plate. Under 35 ℃ ,the Candida was cultured for 24 to 48 hours,and observed growth sit- uation of the bacteria and found the minimum inhibitory concentration（MIC） of drug. Results MIC test of baicalein showed that Candida albicans of MIC was 0. 956 μg/mL,Candida glabrata of MIC was 1. 047μg/mL,Candida tropicalis and Portugal Candida of MIC were 1. 138 μg/mL,Candida krusei and Trichosporon yeast of MIC were 1. 411 μg/mL. Conclusion Inhibitory effect in vitro of baicalein on Candida albicans was best,and the second was Candida glabrata,Candida tropicalis and Portugal Candida were bad, Candida krusei and Trichosporon yeast were worst.

关 键 词：黄芩苷元 最低抑菌浓度 甘肃 酵母菌 

分 类 号：R285.5[医药卫生—中药学]