血液中miRNA用于结直肠癌早期诊断的前期研究  

Preliminary study of miRNA on early diagnosis of colorectal cancer

作　　者：全天一[1] 徐学虎[1] 伍尚标[1] 林彤[1] 江庆萍[1] 刘海波[1] 孙嫣[1] 

机构地区：[1]广州医学院第三附属医院肿瘤科,广东广州510150

出　　处：《临床军医杂志》2013年第4期355-356,共2页Clinical Journal of Medical Officers

基　　金：广东省卫生厅医学科研课题(A2011277);广州市科技和信息化局科技支撑计划项目(2010J-E141);2012年广东省中医药局科研课题(20121134);2011年广东省部产学研结合引导项目(2011B090400526;粤财教[2011]438号)

摘　　要：目的探讨血液中miRNA用于结直肠癌早期诊断的意义。方法选择我院收治的结直肠癌32例,抽取血液与健康体检者的血液对比miRNA。结果肿瘤患者血清中miR-21、miR-31、miR-106a水平明显高于正常人,miR-135a、miR-135b、miR-143、miR-145水平明显低于正常人(P<0.05);肿瘤患者血清中miR-31水平Ⅳ期、Ⅲ期明显高于Ⅰ期(P<0.05)。结论 miRNA可反映结直肠癌的发生,说明miRNA可作为新的生物标记物用于结直肠癌的早期诊断。Objective To investigate the significance of miRNA in the early diagnosis of colorectal cancer. Methods Blood samples were extracted from 32 patients with colorectal cancer, and then their miRNA level was compared with that from healthy subjects. Results The levels of miR-21, miR-31 and miR-106a were significantly higher and that of miR-135a, miR-135b, miR-143 and miR-145 were significantly lower in the tumor sera than in the normal controls＇ （P 〈 0.05 ）. In the tumor sera, the level of miR-31 at Phase IV or m was significantly higher than that at phase I （P 〈 0.05 ）. Conclusion The level of miRNA can reflect the incidence of colorectal cancer, ancl can be used as a biomarker for the early diagnosis of colorectal cancer.

关 键 词：结直肠肿瘤 早期诊断 

分 类 号：R735.35[医药卫生—肿瘤]