新城疫病毒HBNU/LSRC/F3株与Mukteswar、LaSota株抗肿瘤效果的比较  

Comparison of antitumor effect of Newcastle disease virus HBNU/LSRC/F3 strain to ECA109 cells with Mukeswar and LaSota strains

作　　者：高翠[1] 王静[1] 马媛媛[1] 张斌[2] 邢立强[1] 刘华[1] 刘进军[1] 刘开扬[1] 

机构地区：[1]河北北方学院生命科学研究中心,河北张家口075000 [2]河北北方学院附属第一医院检验科,河北张家口075000

出　　处：《中国肿瘤生物治疗杂志》2013年第2期212-216,共5页Chinese Journal of Cancer Biotherapy

基　　金：河北省自然科学基金资助项目(No.C2010001450)~~

摘　　要：目的:研究新城疫病毒(Newcastle disease virus,NDV)弱毒力株HBNU/LSRC/F3对人食管癌ECA109细胞凋亡的影响,并与其他两株NDV(弱毒力株LaSota株、中等毒力Mukteswar株)的抗肿瘤作用进行比较。方法:体外培养ECA109细胞,不同NDV经扩增后分别感染ECA109细胞,CCK-8法检测各NDV对ECA109细胞增殖的抑制作用,激光共聚焦显微镜观察经各NDV感染后ECA109细胞的凋亡情况,流式细胞术检测其早期细胞凋亡,DNA琼脂糖凝胶电泳检测其晚期凋亡情况。结果:HBNU/LSRC/F3株NDV能抑制ECA109细胞的增殖,且强于LaSota株,但稍弱于Mukteswar株(P<0.05)。ECA109细胞感染5×10-4Hu/ml NDV时,HBNU/LSRC/F3株、Mukteswar株、LaSota株对ECA109细胞的增殖抑制率分别为(31.43±1.57)%、(39.87±1.99)%和(19.89±0.99)%。流式细胞仪检测结果显示,HBNU/LSRC/F3株感染后ECA109细胞的早期凋亡率为(21.32±0.44)%,而Mukteswar株和LaSota株的早期凋亡率为(22.27±0.23)%和(14.32±0.61)%;激光扫描共聚焦显微镜和DNA琼脂糖凝胶电泳检测结果显示,Mukteswar株以诱导晚期凋亡为主,HBNU/LSRC/F3株以诱导ECA109细胞早期凋亡为主,且较LaSota株明显。结论:弱毒力HBNU/LSRC/F3株可有效抑制ECA109细胞增殖,诱导ECA109细胞早期凋亡,虽略低于中等毒力Mukteswar株,但远高于弱毒力LaSota株,因此HBNU/LSRC/F3株可能具有更高的抗肿瘤临床应用价值。Objective：To explore the impact of Newcastle disease virus （lentogenic HBNU/LSRC/F3 ） strain on apop- tosis of esophageal cancer ECA109 cells, and to compare with the other two strains （ lentogenic LaSota strain and mesogen- ic Mukteswar strain） of their anti-tumor effects. Methods： ECA109 cells were cultured in vitro and infected by three NDV strains respectively. The proliferation inhibiting effects of NDVs on ECA109 cells were detected by CCK-8. The apoptosis of ECA109 ceils after NDV infection was detected by laser confocal microscopy. Flow cytometry and agarose gel electro- phoresis were used to detect the early and late apoptosis of ECAI09 cells, respectively. Results： HBNU/LSRC/F3 NDV strain suppressed the proliferation of ECA109 cells, which was stronger than LaSota strain and weaker than Mukteswar strain （P 〈 0.05 ）. The proliferation inhibition rates of HBNU/LSRC/F3 strain, Mukteswar strain and LaSota strains on ECAI09 cells were （39.87 ± 1.99） %, （31.43 ± 1.57 ） % and （ 19.89 ± 0.99） %, respectively, infected by 5 x 10 -4 Hu/ml NDV. Flow cytometry showed that the early apoptosis rate of ECA109 cells infected by HBNU/LSRC/F3 strain was （21.32 ±0.44） %, while Mukteswar strain was （22.27 ± 0.23 ） % and LaSota strain was （ 14.32 ± 0.61 ） %. Laser scanning confocal microscopy and DNA gel electrophoresis showed that Mukteswar strain primarily induced the late apopto-sis. Whereas, HBNU/LSRC/F3 strain primarily induced the early apoptosis, which was more obvious than LaSota strain. Conclusion： Lentogenic HBNU/LSRC/F3 strain can effectively inhibit ECA109 cell proliferation and induce early apopto- sis of ECA109 cells, which was lower than Mukteswar strain and significantly higher than LaSota strain. Therefore, HB- NU/LSRC/F3 strain may have a high clinical value for anti-tumor therapy.

关 键 词：新城疫病毒 HBNU/LSRC/F3株 ECA109细胞 凋亡 

分 类 号：R730.54[医药卫生—肿瘤] R735.1[医药卫生—临床医学]