Tyrosol和Farnesol对白念珠菌生物被膜形成作用的基因表达谱研究  

Study on the transcription profiling of biofilm formation by Candida albicans at different phases exposed to tyrosol and farnesol

作　　者：谭军艳[1] 张琰[1] 虞丽华[1] 马鸣[1] 陈晓君[1] 魏昕[1] 

机构地区：[1]南京医科大学口腔医学研究所,210029

出　　处：《中华微生物学和免疫学杂志》2013年第4期258-264,共7页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金（81271151）;江苏省高校优势学科建设工程资助项目

摘　　要：目的研究Tyrosol和Famesol对不同时相白念珠菌生物被膜形成的调控机制。方法研究对象为白念珠茵标准株SC5314。设立Tyrosol处理组、Farnesol处理组、Tyrosol和Famesol联合处理组、对照组。生物被膜6h和24h收集细胞，提取RNA，反转录cDNA。cDNA与白念珠菌全基因组8K表达谱芯片杂交。采用LuxSean双通道激光扫描仪进行扫描。LttxSean3．0图像分析软件对芯片图像进行分析。借助KEGG基因库对芯片数据进行生物信息学分析。结果不同生物被膜时相，Tyrosol和Famesol对白念珠菌基因表达调控作用不同。Tyrosol和Farnesol调控生物被膜形成相关基因、菌丝相基因和酵母相基因。Tyrosol是生物被膜基因表达的正向调控效应因子。Farnesol是负向调控效应因子。Tyrosol和Famesol主要调控的基因包括生物学过程、分子功能及细胞成分相关基因，这些基因主要通过参与物质代谢通路调控白念珠菌生物学特点及生物被膜形成。结论Tyrosol和Famesol通过调控白念珠菌哇物被膜形成相关基因调控毕物被膜形成。Objective To investigate the effects of tyrosol and farnesol on the transcription profiling of C. albicans biofilm by microarray analysis. Methods The standard strain of C. albicans, SC5314 were cultured into four groups （ tyrosol treated, farnesol treated, tyrosol and farnesol co-treated, and untreated control）. The cell suspensions of SC5314 were prepared and dispensed into polystyrene flasks to form biofilm. Then, the biofilms were collected at 6 h and 24 h respectively after culturing. RNA samples were extracted and synthesized into eDNA through reverse transcription. The genome arrays were scanned with a confocal LuxScanTM scanner and the images were then analyzed by using LuxSeanTM 3.0 software （both from CapitalBio）. Bioinformaties analysis of the data was carried out by comparatively analyzing S. cerevisiae gene in KEGG gene database. Results The eDNA microarray data showed that tyrosol and famesol regulated biofilm formation by regulating the genes associated with biofilm formation such as hypha genes and yeast genes. Tyrosol positively regulated gene expression of C. albicans biofilm, while farnesol played negative role. There were striking differences in gene expression patterns between tyrosol or farnesol treated groups and the control. Tyrosol had no antagonistic effect to farnesol. Bioinformatics analysis showed that the differential gene expression was involved in biological process, molecular function process and cell component formation. These genes regulated the C. albicans biofilm formation by encoding proteins involved in the biological metabolism. Conclusion Tyrosol and famesol influenced the formation of C. albicans biofilm through regulating gene expression which showed differences at different phases of biofilm formation.

关 键 词：白念珠菌生物被膜 TYROSOL FARNESOL 基因芯片 

分 类 号：R3[医药卫生—基础医学]