超声内镜探头引导下乙醇瘤内注射治疗胰腺癌的实验研究  

EUS-guided percutaneous intra-tumor injection of ethanol on orthotopic human pancreatic cancer model in mice

作　　者：张文颖[1] 吴红玉[1] 郭杰芳[1] 郭妍[1] 刘晓[1] 龚艳芳[1] 高军[1] 金震东[1] 李兆申[1] 

机构地区：[1]第二军医大学长海医院消化内科,上海200433

出　　处：《中华消化内镜杂志》2013年第4期185-188,共4页Chinese Journal of Digestive Endoscopy

基　　金：基金项目：上海市科学技术委员会资助（11D21921605）

摘　　要：目的探讨超声内镜探头引导下乙醇瘤内注射治疗胰腺癌的疗效。方法原位胰腺癌裸鼠20只随机表法随机分为2组，实验组行95％乙醇注射治疗，对照组注射生理盐水，比较治疗前及治疗后24h血清淀粉酶变化。分别于治疗前及治疗后第7天行超声内镜探头测量肿瘤大小，计算相对肿瘤体积和相对肿瘤增殖率。光学显微镜下观察治疗前及治疗1周后肿瘤组织病理改变。结果与治疗前相比，实验组治疗后24h血清淀粉酶出现轻度增高[（2873．5±958．9）U／L比（2004．3±402．0）U／L，P〉0．05]，但与对照组治疗后24h的[（2066．1±396．6）U／L]比较，差异无统计学意义（P=0．061）。实验组治疗后第7天相对肿瘤体积明显低于对照组[（0．45±0．08）比（1．25±0．06），P〈0．01）]，相对肿瘤增殖率为36％。治疗1周后实验组病理组织学显示肿瘤坏死变性严重，有大面积凝固性坏死；而对照组仅见少量坏死。结论95％乙醇瘤内注射可造成肿瘤坏死，在裸鼠原位胰腺癌模型中有一定的治疗效果。Objective To study the efficacy of EUS-guided percutaneous ethanol intra-tumor injection on orthotopic human pancreatic cancer mice model. Methods Twenty nude mice with orthotopic transplantation of human pancreatic cancer were randomly divided into 2 groups to receive 95% ethanol intratumor injection or normal saline intra-tumor injection. The serum amylase levels were detected before and 24h after treatment. Tumor sizes were measured by high-frequency EUS （75 MHz） before and 7d after treatment. Relative tumor volume and relative rate of tumor proliferation were calculated. Histological changes of tumor tissue were observed by an optical microscope before and 7d after treatment. Results The serum amylase in experimental group was slightly elevated 24h after treatment [ （ 2873.5 ± 958.9 ） vs. （ 2004. 3 ± 402. 0）,P〉0. 05] ,but no significant difference was seen when compared with that of the control group （2066. 1 ±396. 6, P =0. 061 ）. Relative tumor volume at 7th day after injection in experimental group was significantly lower than that of control group （0. 45 ±0. 08 vs. 1.25 ±0. 06,P 〈0. 01 ）. The relative rate of tumor proliferation at 7th day after injection was 36% in experimental group. The massive coagulation necrosis was seen at 7th day after injection in experimental group, while only minor necrosis was observed in control group. Conclusion EUS-guided percutaneous intra-tumor injection of 95% ethanol can lead to tumor necrosis, and have a therapeutic effect in orthotopic pancreatic cancer nude model.

关 键 词：胰腺癌 内窥镜超声检查 乙醇 注射 动物实验 

分 类 号：R735[医药卫生—肿瘤] R44[医药卫生—临床医学]