整合素β1调控成釉细胞外基质蛋白表达的研究  

Integrin β1 gene regulates extracellular matrix protein expression of ameloblasts

作　　者：张海英[1] 邵丹 李伯翰[3] 韩婷婷[3] 李东亮[3] 王玉敏[3] 孙岩[1] 张娟娟[1] 高玉光[3] 

机构地区：[1]潍坊医学院口腔医学院 [2]青岛经济技术开发区第一人民医院 [3]滨州医学院附属医院口腔科

出　　处：《牙体牙髓牙周病学杂志》2013年第5期287-290,309,共5页Chinese Journal of Conservative Dentistry

基　　金：国家自然科学基金(30973327);山东省自然科学基金(ZR2010HM076))

摘　　要：目的:观察整合素β1(Integrinβ1)在小鼠牙胚成釉细胞中的表达及其对成釉细胞细胞外基质蛋白——釉原蛋白(Amelogenin,AMELX)、成釉蛋白(Ameloblastin,AMBN)、釉成熟蛋白(Amelotin,AMTN)mRNA表达的影响。方法:采用免疫组化方法检测整合素β1在小鼠牙胚成釉细胞中的表达。构建真核重组表达载体pcDNA3.1/myc-hisA-Integrinβ1,并将其转染至小鼠成釉细胞,利用实时定量Real-time PCR法检测AMELX、AMBN、AMTN mRNA表达水平。结果:整合素β1在小鼠牙胚发育各期成釉细胞中均有表达。RT-PCR检测显示,与转染空白质粒的对照组相比,转染pcDNA3.1/myc-hisA-Integrinβ1的成釉细胞中AMELXmRNA的表达水平明显上调(P<0.05),但对AMBN和AMTN mRNA表达无显著促进作用(P>0.05)。结论:整合素β1在小鼠牙胚成釉细胞中表达,并促进Amelogenin在成釉细胞中的表达,提示整合素β1通过调节釉原蛋白而影响牙齿的发育和矿化。AIM： To observe intergrinβ1 expression in mouse tooth germ ameloblasts and to examine its effects on mRNA expression of extracellular matrix proteins including amelogenin （ AMELX）, ameloblastin （AMBN） and amelotin （AMTN）. METHODS： Intergrin[31 protein expression in mouse tooth germ ameloblasts was examined by immunohistochemical method. Plasmid pcDNA3. 1/myc-hisA-Integrin[M was constructed and transfected into mouse tooth germ ameloblasts. AMELX, AMBN and AMTN mRNA expression of the ceils was detected by real-time RT-PCR. RESULTS： Integrin[31 was expressed in ameloblasts in the whole process of mouse tooth germ develop- ment. AMELX mRNA in ameloblasts was significantly increased by the transfection of pcDNA3. 1/myc- hisA - Integrinβ1 （ P 〈 0.05 ）, While AMBN and AMTN mRNA was not significantly affected （ P 〉 0.05 ）. CONCLUSION ： Integrin[31 might play an important role in tooth development and mineralization by regulation of amelogenin expression.

关 键 词：整合素Β1 成釉细胞 AMELX 成釉细胞细胞外基质蛋白 

分 类 号：R780.2[医药卫生—口腔医学]