Hey1基因过表达对小鼠单核细胞RAW264.7向破骨细胞分化的影响  

The effect of hey1 overexpression on osteoclastogenesis capacity of murine monocytes RAW264.7

作　　者：许波[1,2] 殷霄[1] 张安生[1] 柴雪[1] 王蕾[1] 孙汉堂[1] 张亚庆[1] 

机构地区：[1]第四军医大学口腔医学院,陕西西安710032 [2]解放军第37医院,四川雅安625000

出　　处：《牙体牙髓牙周病学杂志》2013年第5期291-293,共3页Chinese Journal of Conservative Dentistry

基　　金：国家自然科学基金资助项目(81070832)

摘　　要：目的:观察Hey1基因过表达对小鼠单核细胞RAW264.7向破骨细胞分化的影响。方法:用Lipofectamine 2000将Hey1表达质粒转染RAW264.7细胞系;10μg/mL Blasticidin筛选稳定转染的细胞克隆,RT-PCR检测Hey1基因表达水平;用RANKL诱导RAW264.7细胞向破骨细胞分化,抗酒石酸磷酸酶(TRAP)染色后进行破骨细胞计数。结果:Blasticidin筛选出的细胞株Hey1 mRNA表达水平显著高于正常RAW264.7细胞;在50 ng/mL RANKL诱导条件下,Hey1过表达的RAW264.7细胞分化形成的破骨细胞数显著低于正常RAW264.7细胞(P<0.05)。结论:Hey1具有抑制小鼠单核细胞RAW264.7向破骨细胞分化的作用。AIM ： To observe the effect of Heyl overexpression on the differentiation of murine monocytes of RAW264.7 into osteoelasts. METHODS ： Expression plasmid carrying Hey1 mRNA encoding sequence was transfected into RAW264.7 cells by lipofectamine 2000. Stably transfected cells were screened by blasticidin at concentration of 10μg/mL. Heyl mRNA expression was analyzed by RT-PCR. RANKL was used to induce RAW264.7 cells to differentiate into osteoelasts. The osteoclasts were counted after tartrate-resistant acid phosphatase （TRAP） staining. RE- SULTS ： Hey1 mRNA expression level of the cell line screened by blastieidin was significantly higher than that of parental RAW264.7 Cells. In the presence of 50ng/ml RANKL, more osteoclasts were doserved in the Heyl over expressed RAW 264.7 cells than in the parental RAW264.7 cell （ P 〈 0.05 ）. CONCLUSION ： Hey1 has an inhibitory effect on the differentiation of murine RAW264.7 monocytes into osteoclasts.

关 键 词：Hey1 RAW264 7 破骨细胞 TRAP染色 

分 类 号：R780.2[医药卫生—口腔医学]