载银二氧化钛纳米管涂层种植体体内生物相容性研究  

In vivo biocompatibility evaluation of titanium dental implant with surface modified by Ag-loaded TiO_2 nanotube array

作　　者：刘梦园[1] 巴亚玛[1] 张程程[1] 梅盛林[1] 马千里[1] 张玉梅[1] 

机构地区：[1]第四军医大学口腔医学院,陕西西安710032

出　　处：《牙体牙髓牙周病学杂志》2013年第5期303-309,共7页Chinese Journal of Conservative Dentistry

基　　金：国家自然科学基金项目(81070862)

摘　　要：目的:观察载银二氧化钛(TiO2)纳米管涂层种植体的体内生物相容性,探讨不同表面涂层体内异物反应(Foreign Body Response,FBR)程度。方法:分别对纯钛抛光组(PT)、TiO2纳米管组(NT)、低浓度载银TiO2纳米管组(NAg-L)和高浓度载银TiO2纳米管组(NAg-H)种植体的表面形貌进行扫描电镜观察。将4组种植体(各12枚)植入SD大鼠背部皮下,饲养12 d后取材。采用免疫荧光染色计数测量纤维包裹层内CD68阳性的巨噬细胞密度;组织学染色观察纤维包裹层厚度;实时定量PCR分析周围组织巨噬细胞趋化蛋白(Monocyte chemoattractant protein,MCP-1)的表达量。结果:纤维包裹层内巨噬细胞密度、各组周围组织中MCP-1 mRNA表达量均以PT组最多,与其他各组相比均有统计学意义(P<0.05);而NAg-L、NAg-H、NT 3组间无统计学差异(P>0.05);各组包膜厚度为NT组<NAg-L组<NAg-H组<PT组。结论:低浓度银纳米颗粒载入不增强材料的异物反应,高浓度银轻微增强其异物反应。AIM： To observe the in vivo biocompatibility of titanium dental implant with surface modified by Agloaded TiO2 nanotube array. METHODS： The implant surface was modified by polished titanium（PT）, TiO2 nanotube array（ NT）, Ag-loaded TiO2 nanotube array with low density（ NAg-L ） and Ag-loaded TiO-2 nanotube array with high density（NAg-H） respectively. The surface morphology of the samples in the 4 groups was examined by SEM. Then 12 implants of each group were subcutaneouly transplantated into the back of SD rats. 12 d after transplantation foreign body reaetion（FBR） was assessed by quantification of CD68-positive macrophages in the capsule and by measuring the reactive capsule thickness. The mRNA expression of monocyte chemoattraetant protein-1 （ MCP-1 ） was measured by RT-PCR. RESULTS ： The density of macrophages and MCP-1 mRNA expression were significantly high- er in PT group than in other groups （P 〈0.05）. However, there was no statistical difference among NAg-L, NAg-H and NT groups（ P 〉 0.05 ）. Reactive capsule thickness was in the order of NT group 〈 NAg-L group 〈 NAg-H group 〈 PT group. CONCLUSION： Agloaded TiO-2 nanotube array with low density does not raise the foreign body reaction, however high density slightly enhances the foreign body reaction.

关 键 词：生物相容性 异物反应 载银 纳米管 

分 类 号：R783.1[医药卫生—口腔医学]