Ti-6Al-7Nb合金表面经HF酸蚀+阳极氧化处理后对骨髓间充质干细胞特性影响的研究  

Effects of HF and anodic oxidation treated Ti-6Al-7Nb alloy on rat bone marrow mesenchymal stem cells

作　　者：张述寅[1] 胡开进[1] 莫静珍[1] 李永锋[1] 沈舒宁[1] 高麒[1] 孔亮[1] 

机构地区：[1]第四军医大学口腔医学院,陕西西安710032

出　　处：《牙体牙髓牙周病学杂志》2013年第5期310-314,326,共6页Chinese Journal of Conservative Dentistry

基　　金：国家自然科学基金(30900284;31100668);陕西省社发攻关项目(2011K14-03-01)

摘　　要：目的:观察HF酸蚀+阳极氧化处理后Ti-6Al-7Nb表面形貌、化学成分以及对骨髓间充质干细胞(BMSCs)生物学特性的影响。方法:纯钛和Ti-6Al-7Nb样本各30个,碳化硅砂纸打磨抛光表面后,各随分对照组(n=15),对照组不作处理;处理组进行HF酸蚀+阳极氧化。分别用扫描电镜(SEM)、原子力学显微镜(AFM)和X线能谱仪(XPS)观察4组样本表面形貌,分析表面化学成分。将体外培养的第4代SD大鼠BMSCs接种于各组合金表面,SEM观察细胞形貌;MTT方法检测细胞在材料表面的粘附和增殖;ALP试剂盒检测细胞ALP活性。结果:纯钛和Ti-6Al-7Nb经HF酸蚀+阳极氧化处理后表面有均匀分布的纳米管状结构,表面凹凸不平;XPS检测发现表面出现氟(F)化物,铝(Al)元素主要以Al2O3的稳定形式存在。BMSCs在两处理组表面粘附、增殖及ALP活性均显著高于其相应对照组(P<0.05);两材料间相比,Ti-6Al-7Nb处理组ALP活性显著高于纯钛处理组(P<0.05)。结论:Ti-6Al-7Nb经HF酸蚀+阳极氧化处理后,可以促进BMSCs的粘附、增殖和ALP活性。AIM： To investigate the surface morphology and chemical composition of HF acid etch and anodic oxidation modified Ti--6A1-7Nb alloy and to observe the effects of surface modification on the biological characteristics of rat bone marrow mesenchymal stem cells（BMSCs）. METHODS： 30 Ti samples and 30 Ti-6A1-7Nb samples were used. 15 pure Ti and 15 Ti-6A1-7Nb samples were treated by HF acid etch and anodic oxidation, another 15 of each brand were untreated as the controls. The morphology of the samples was observed by SEM and atomic force microscope （AFM）. The chemical composition was examined by X-ray photoelectron spectroscopy （XPS）. Rat BMSCs were cultured on the samples and the cell morphology was observed by SEM. Cell adhesion and proliferation rate were tested by MTT assay. Alkaline phosphatase （ALP） activity of BMSCs was determined by ALP kit. RESULTS ： The treated surface exhibited uniform nanotube array and the main chemical composition was titania with trace fluorine and aluminum. HF acid etch and anodic oxidation treatment significantly enhanced BMSCs adhesion, proliferation and ALP activity of both pure Ti grouD and Ti-6A1-7Nb alloy groups C P 〈 0.05）. Modified Ti -6A1 -7Nb alloy group showed significantly higher ALP activity than modified pure Ti groups （P 〈 0.05 ）. CONCLUSION ： HF acid etch and anodic oxidation modified Ti-6A1-7Nb alloy surface may promote adhesion, proliferation and ALP activity of BMSCs on the surface.

关 键 词：Ti-6Al-7Nb 阳极氧化 酸蚀 骨髓间充质干细胞 

分 类 号：R783.1[医药卫生—口腔医学]