人正常和感染牙髓组织中矿化因子表达的研究  

Expression of minaralization-related genes in normal and inflamed human dental pulp tissues

作　　者：王海婧[1] 白玉[1] 王亚飞[1] 蒋文凯[1] 贾谦[1] 倪龙兴[1] 

机构地区：[1]第四军医大学口腔医学院,陕西西安710032

出　　处：《牙体牙髓牙周病学杂志》2013年第5期334-337,共4页Chinese Journal of Conservative Dentistry

基　　金：国家自然科学基金资助(81770946)

摘　　要：目的:观察不同状态牙髓组织矿化相关基因表达情况,以探讨矿化相关基因在牙髓损伤修复过程中的变化。方法:收集临床新鲜拔除的成人恒牙牙髓,荧光实时定量检测正常、浅龋、中龋、深龋、牙髓炎时牙髓组织中碱性磷酸酶(alkaline phosphatase,ALP)、骨钙蛋白(osteocalcin,OCN)、转录因子(runt-relatedtranscription factor 2,Runx2)各基因的相对表达量。结果:与正常组织相比ALP mRNA在中龋及深龋组牙髓中表达量升高(P<0.05);OCN mRNA在深龋牙髓中表达量高(P<0.05);Runx2 mRNA在浅、中、深龋牙髓中的表达无明显变化(P>0.05)。但以上3种基因在牙髓炎牙髓中的表达均较正常组明显下降(P<0.05)。结论:在正常牙髓组织中矿化相关因子均有表达,ALP参与牙髓组织防御修复反应,OCN参与中晚期牙髓修复反应,牙髓炎后期,矿化因子作用减弱。AIM： To investigate the expression of minaralization-related genes in normal and inflamed human dental pulp tissues. METHODS： Dental pulp from freshly extracted teeth for orthodontic reasons was collected. Total RNA of human dental pulp was extracted. Expression of minaralization-related genes, including alkaline phosphatase （ALP） , osteocalcin （OCN） and runt-related transcription factor （Runx）-2 in normal and inflamed dental pulp tissues were detected respectively by RT-q PCR. RESULTS： Higher expression of ALP was observed in the dental pulp of the teeth with moderate and deep caries （ ompared with the normal group, P 〈 0.05）. Higherst OCN ex- pression was found in the pulp tissue of the teeth with deep caries（ P 〈 0.05 ）. Runx2 expression in healthy dental pulp of healthy teeth, the teeth with shallow, moderate or deep caries was not significantly defferent （P 〉 0.05 ）. However, the mRNA e^pression of the three genes decreased in pulp tissures with irreversible pulpitis （P 〈 0.05 ）. CONCLU- SION： The mineralization refated genes expresse in normal dental pulp tissues. ALP may have a potential role in the early stage of reparative dentin formation, while OCN may be related to pulp reaction in the middle and late stage of pulpitis. At late stage of infection the fuction of mineralization - related genes may decrease.

关 键 词：龋病 牙髓炎 荧光实时定量PCR OCN ALP RUNX-2 

分 类 号：R780.2[医药卫生—口腔医学]