髓样抑制细胞在自身免疫性肝炎小鼠模型中的变化及其意义  

Alteration of myeloid-derived suppressor cells in a mouse model of autoimmune hepatitis and its significance

作　　者：范舒萍[1] 王艳红[1] 赵永华[2] 米洋[1] 侯星生[1] 王姝月[1] 邓志华[3] 罗旭光[1] 王桂琴[1] 

机构地区：[1]山西医科大学微生物学与免疫学教研室,太原030001 [2]太原钢铁(集团)有限公司太钢总医院体检中心,030008 [3]山西医科大学第二附属医院消化科,太原030001

出　　处：《免疫学杂志》2013年第5期387-390,共4页Immunological Journal

基　　金：山西省科技攻关项目(20120313024-3)

摘　　要：目的探讨髓样抑制细胞(myeloid-derived suppressor cells,MDSCs)在自身免疫性肝炎(autoimmune hepatitis,AIH)发生发展中的作用。方法用S-100建立C57BL/6小鼠AIH模型,随机将小鼠分为模型组和对照组,每组15只。从模型组和对照组C57BL/6小鼠的肝脏和骨髓中分离单个核细胞,用流式细胞术检测MDSCs数量变化。结果 CD11b+MDSCs在模型组小鼠的肝脏和骨髓中数量高于对照组(P<0.05),并且在骨髓中增高的比例显著大于肝脏,CD14+的细胞数量在模型组小鼠低于对照组的数量(P<0.05)。结论 AIH发生发展中MDSCs参与了局部的免疫调节作用。For investigating the role of myeloid-derived suppressor cells （MDSCs） in the development of autoimmune hepatitis （AIH）, C57BL/6 mice were randomly divided into model group and control group, with 15 mice in each group in this research. AIH mouse model was established in C57BL/6 mice by using S-100. In model group and control group, mononuclear cells were separated from liver and bone marrow of mice, and analyzed by flow cytometry. The results showed the number of CDllb＋ MDSCs in liver and bone marrow of AIH group were higher than those in the control group separately （P〈 0.05）, and the proportion of increased CD111b＋ MDSCs in bone marrow was significantly higher than those in liver. In the mean time, the count of CD14＋ cells in AIH mice were less than that in control group （P〈 0.05）. In conclusion, MDSCs may play a role in the development of AIH in local immunity.

关 键 词：髓样抑制性细胞 自身免疫性肝炎 CD11B CD14 

分 类 号：R575.1[医药卫生—消化系统]