巨噬细胞移动抑制因子促进肿瘤进展并影响胶质瘤预后  

Cytokine MIF contributes to the tumor progression and influences the prognosis of the patients with primary glioma

作　　者：江晓珍[1] 王小冰[2] 邓葵[3] 郑炎[4] 廖冰[1] 李斌[1] 李智[1] 

机构地区：[1]中山大学附属第一医院病理科,广州510080 [2]广东省人民医院病理科 [3]南京医科大学附属常州市第二人民医院骨科 [4]安徽省巢湖市第一人民医院病理科

出　　处：《中国神经精神疾病杂志》2013年第4期236-240,共5页Chinese Journal of Nervous and Mental Diseases

基　　金：广东省科技计划项目(编号:2010B060900104;2012A030400008)资助

摘　　要：目的探讨巨噬细胞移动抑制因子(macrophage migration inhibitory factor,MIF)、细胞增殖指数Ki-67、微血管增生密度CD34在胶质瘤组织中的表达及对临床预后的影响。方法收集具有明确病理分级的94例新鲜胶质瘤标本,依据《WHO中枢神经系统肿瘤组织学分类》指导,低级别胶质瘤(Ⅰ-Ⅱ级)43例,高级别胶质瘤(Ⅲ-Ⅳ级)51例。采用免疫组织化学方法检测94例胶质瘤组织中MIF、Ki-67和CD34的表达,采用Ka plan-Meier、Cox比例风险回归模型分析影响患者的累积生存率及预后的相关因素。结果胶质瘤组织中MIF、Ki-67、CD34的表达水平随肿瘤级别的增加而增加,肿瘤组织内MIF表达水平与肿瘤组织学分级、Ki-67、CD34表达均密切相关(χ2=16.779,P<0.05;χ2=26.405,P<0.05;t=-5.340,P<0.05)。Kaplan-Meie分析结果显示肿瘤组织学分级、MIF、Ki-67、CD34的表达水平及发病年龄与临床预后密切相关(P均<0.05),并且经Cox比例风险回归模型分析,组织学分级与细胞增殖指数Ki-67是影响胶质瘤患者预后的独立危险因素(P均<0.05)。结论MIF可能通过促进细胞增殖、诱导肿瘤血管新生参与胶质瘤的发展和演进,与患者临床预后密切相关,针对MIF的靶点治疗有望成为胶质瘤的有效治疗方案。Objective To investigate the expression of MIF, proliferation index （Ki-67）, microvessel density （MVD） in gliomas and their influence on the prognosis of patients with primary glioma. Methods Ninety-four fresh glioma specimens with a defined histological grade including 43 of lower histological grading（WHO grades I and II）, 51 of higher histological grading（WHO grades III and IV） . Immunohistochemical study for MIF, CD34 and Ki-67 were performed on paraffin-embedded tissues of 94 cases of gliomas. Kaplan-Meier and multivariate analysis of Variance was used to analysis the survival rate of patients with gliomas and to estimate the asssociation independent risk factors for prognosis of glioma, respectively. Result The expression levels of MIF, proliferation index （Ki-67） and microvessel density （MVD） were increasing in higher histological grading （WHO grades III and IV）. Meanwhile, the expression of MIF was significantly associated with histological grading, Ki-67 and MVD （X^2=16.779, P〈0.05;X^2=26.405, P〈0.05; t=-5.340, P〈0.05 ）. Univariate analysis revealed that the histological grading, expression of MIF, proliferation index and MVD were closely associated with the prognosis of patients with primary gliomas （both P〈0.05）. However, only histological grading and Ki-67 index were the independent risk factors for prognosis of glioma （both P〈0.05）. Conclusion MIF contributes to the progression of glioma and influences the prognosis of patients with gliomas. The study suggests that MIF might be a valuable predictive factor for prognosis of zliomas and might be a possible novel target for molecular treatment of gliomas.

关 键 词：胶质瘤 巨噬细胞移动抑制因子(MIF) 增殖 预后 

分 类 号：R739.4[医药卫生—肿瘤]