亚砷酸联合顺铂对人鼻咽癌裸鼠移植瘤的抑制作用及对DAPK的影响  

Inhibitory effect of arsenic trioxide combined with cisplatin on human nasopharyngeal carcinoma xenograft and DAPK in nude mice

作　　者：黄雪琴[1] 王晓刚[1] 胡俊丽[2] 周慧[2] 周克元[1] 张月飞[2] 

机构地区：[1]广东医学院,广东湛江524023 [2]广东医学院附属医院耳鼻咽喉科

出　　处：《临床耳鼻咽喉头颈外科杂志》2013年第9期479-483,共5页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：广东省科技计划项目;湛江市科技攻关计划项目(No:2011C3109010);广东医学院青年基金(No:Q2010020)

摘　　要：目的:研究亚砷酸(As2O3)联合顺铂(DDP)对人鼻咽癌CNE-2Z裸鼠移植瘤的生长抑制作用及对死亡相关蛋白激酶(DAPK)的影响。方法:建立人鼻咽癌裸鼠移植瘤模型,随机分为对照组、As2O3组、DDP组和As2O3加DDP组,比较各组的抑瘤作用;并对标本分别进行光镜、原位末端标记(TUNEL)检测,采用实时荧光定量PCR法与免疫组织化学方法检测DAPK的表达。结果:As2O3和As2O3加DDP均能显著抑制人鼻咽癌裸鼠移植瘤的生长,诱导肿瘤细胞凋亡,并能上调DAPK的表达。结论:As2O3可抑制人鼻咽癌CNE-2Z裸鼠移植瘤的生长,该作用与诱导人鼻咽癌CNE-2Z裸鼠移植瘤细胞凋亡、上调DAPK的表达相关。As2O3与DDP联用可起协同作用。Objective：To study the inhibitory effect of Arsenic Trioxide（As203 ） combined with diamminedi- chloroplatinum（DDP） on the growth of human nasopharyngeal carcinoma cell strain CNE-2Z xenograft in nude mice, and to explore the possible effect mechanisms of the antitumor. Method：The models of human poorly differ- entiated nasopharyngeal carcinoma in nude mice were established and randomly divided into four groups, control group, As2O3 group, DDP group and As2O3 ＋DDP group. The effect of antitumor on each group was studied. The specimen obtained from the mice were detected by opticalmicroscope and tdt-mediated dutp rock end labeling （tuned method . Expression of DAPK was detected by real time-PCR and immunohistochemistry. Result： Asz Oa group and As2O3 ＋DDP group could obviously inhibit the growth of tumor, induce the apoptosis of human naso- pharyngeal carcinoma cell and up-regulate the expression of RASSF1A. Conclusion： As2O3 can greatly inhibit the growth of human nasopharyngeal carcinoma cell strain CNEr2Z xenograft in nude mice, which were related to the induced apoptosis of human nasopharyngeal carcinoma cell and up-regulated expression of DAPK Combination of As2 03 with DDP seem to be more effective.

关 键 词：亚砷酸 鼻咽癌 死亡相关蛋白激酶 凋亡 裸鼠 

分 类 号：R739.6[医药卫生—肿瘤]