茉莉酸甲酯对胃癌SGC7901细胞生长抑制机制探讨  

Growth inhibitory effects of methyl jasmonate on human gastric cancer cell line SGC7901 invitro

作　　者：董秋菊[1,2] 张建福[1,2] 韩红霞[1] 汪诗卉[1] 高之峰[1] 费素娟[1] 

机构地区：[1]徐州医学院附属医院消化内科教研室,江苏徐州221002 [2]徐州医学院生理学教研室,江苏徐州221002

出　　处：《中华肿瘤防治杂志》2013年第9期641-645,共5页Chinese Journal of Cancer Prevention and Treatment

基　　金：国家自然科学基金(30570671)

摘　　要：目的:探讨茉莉酸甲酯(MJ)对胃癌SGC7901细胞生长的抑制作用及其机制。方法:四甲基偶氮唑(MTT)法检测细胞生长活性;Hoechst 33258荧光染色法观察凋亡细胞核的形态学变化;AnnexinⅤ-FITC/PI双染流式细胞术检测细胞的凋亡率;蛋白质印迹法检测凋亡蛋白Caspase-3和Caspase-9的表达。结果:MTT法显示,MJ对胃癌SGC7901细胞生长有显著抑制作用,且呈剂量-时间依赖关系,0.5、1.0和2.0mmol/L MJ作用6h后,抑制率分别为(7.10±0.84)%、(18.22±1.82)%和(26.89±1.56)%,溶剂对照组为(0.43±0.26)%,顺铂(DDP)组为(10.44±1.17)%,总体比较差异有统计学意义(F=232,P=0.000),各组间比较差异均有统计学意义,P值均<0.01;采用MJ上述3种浓度作用12h后,抑制率分别为(12.44±1.20)%、(32.70±1.29)%和(52.94±2.29)%,溶剂对照组为(0.31±0.41)%,DDP组为(22.60±1.97)%,总体比较差异有统计学意义(F=1 013,P=0.000),各组间比较差异均有统计学意义,P值均<0.01;MJ三种浓度作用24h后,抑制率分别为(17.37±1.95)%、(41.79±4.40)%和(71.93±4.65)%,溶剂对照组为(0.34±0.21)%,DDP组为(27.72±2.45)%,总体比较差异有统计学意义(F=444,P=0.000),各组间比较差异均有统计学意义,P值均<0.01;其中以浓度为2.0mmol/L MJ作用24h后,对SGC7901细胞的抑制率效果最佳。荧光染色法观察MJ能够诱导人胃癌SGC7901细胞株的凋亡。流式细胞检测显示,MJ可促进胃癌SGC7901细胞的凋亡,凋亡率为(68.14±7.91)%;与溶剂对照组(6.98±1.45)%相比,差异有统计学意义,P=0.013。蛋白质印迹法检测显示,MJ可上调凋亡蛋白Caspase-3和Caspase-9的表达;事先采用辣椒素受体拮抗剂(Cap)预处理,MJ的抗胃癌SGC7901细胞的效应可被消除,与MJ单独给药组相比,MJ对癌细胞的生长抑制率下降为(38.79±2.77)%,P=0.05;凋亡细胞明显减少;肿瘤细胞凋亡率降为(39.81±4.57)%,P=0.045;凋亡蛋白Caspase-3和Caspase-9表达也降低。结论:MJ有显著抑制胃癌SGC7901细胞增殖的作用;辣椒素�OBJECTIVE： To explore the growth inhibitory effects of methyl jasmonate（MJ） on human gastric cancer SGC7901 cell line,and investigate its mechanisms. METHODS： The MTT colorimetry was used to detect the growth inhibitory rates of MJ on human gastric cancer SGC7901 cell; Hoechst 33258 fluorochrome staining was employed to observe cell apoptosis; the apoptosis potential of ceils was assessed by flow cytometry with Annexin V-FITC/PI. The expres- sions Caspase-3 and Caspase-9 were detected by Western blott. RESULTS： MJ inhibited the growth of SGC7901 cells in a dose-and time-dependent manner. After exposed to 0.5,1.0 and 2.0 mmol/L MJ for 6 h, the inhibition rates were about （7.10±0.84）% ,（18.22±1.82）% and （26.89±1.56）% respectively,compared with the solvent control group （0.43± 0.26） % and DDP group（10.44 ± 1. 17）%, there was a statistically significant difference on total （F= 232, P= 0. 000） and each group （P〈0.05）. With the same dose MJ for 12 h, the inhibition rates were about （ 12.44± 1.20） %, （32.70 ± 1.29） % and（52.94± 2.29） % respectively. Compared with the solvent control group（0. 31 ±0.41 ） % and DDP group （22.60± 1.97）% ,there was a statistically significant difference on total （F= 1 013, P = 0. 000） and each group （P〈 0.01）,for 24 h,the inhibition rates were about （17.37±1.95）%,（41. 79±4.40）% and （71. 93±4.65）% ,respectively. Compared with the solvent control group（0.34±0.21）% and DDP group（27.72 ± 2.45）%, there was a statistically significant difference on total （F=444,P=0. 000） and each group （P〈0.01）. 2 mol/L MJ after 24 h had the best inhibition rates. Hoechst 33258 fluorochrome staining showed that there were many apoptotic cells in MJ group. The apoptosis rate was（68. 14±7.91） % ,after exposed to 2.0 mmol/L MJ for 24 h,compared with the solvent control group（6. 98±1.45） % （P=0. 013）. The expressions of Caspase-3 and Caspase-9 protein were increased. Above-mentioned inhibito

关 键 词：胃肿瘤 茉莉酸甲酯 辣椒辣素 细胞凋亡 

分 类 号：R735.2[医药卫生—肿瘤]