负压与BMSCs膜片促进细胞定植和体内成骨的探讨  

Application of Negative Pressure System and BMSCs Cell Sheet in Bone Formation in vivo

作　　者：温鑫鑫 周鹂鹂 刘宏伟 

机构地区：[1]同济大学附属口腔医院牙周科 [2]同济大学口腔医学院口腔生物医学及转化医学实验室,上海200092

出　　处：《口腔颌面外科杂志》2013年第2期85-89,共5页Journal of Oral and Maxillofacial Surgery

基　　金：国家自然科学基金资助项目(81271152);上海市科委科技计划项目(074119514)

摘　　要：目的:研究经过负压处理的TCP支架材料与细胞复合后的体内成骨能力。方法:将TCP支架材料负压处理,和骨髓基质细胞(BMSCs)细胞共培养一段时间后,成骨诱导2周,电镜下观察支架材料和细胞复合情况。再将该复合物与BMSCS细胞膜片复合,植入到裸鼠皮下,8周后作组织切片,HE染色和Masson染色,观察其体内成骨能力。结果:负压(有细胞)+膜片组异位移植8周后,内部可见大量胶原纤维和成骨纤维,在团块样组织内部可见大量成骨细胞,并有血管形成。对照组仅有个别细胞密集区,胶原纤维和成骨纤维较少。空白组未见类似情况。结论:经过负压+骨髓基质细胞膜片处理的TCP支架材料,在体内有成骨潜能。Objective： The present study was proposed to investigate in vivo osteogenic potential of bone marrow stromal cells （BMSCs） in negative pressure treated β-Tricalcium phosphate （β-TCP） scaffolds. Methods： TCP was dealt with the syringe system （made for a subatmospheric pressure）, cocultured or not cocultured with BMSCs, and differentiate into osteogenie direction for 2 weeks. BMSCs sheet was packed into TCP scaffolds and embedded subcutaneously in nude mice for 8 weeks. Animals were sacrificed, osteogenic potential were observed histologically by HE, and Masson stain. Results： TCP cocuhured with BMSCs packed with cell sheets showed better osteogenic potential in vivo . Conclusion： Because of the formation of extracellular matrix, the negative pressure and cell sheets may promote cell colonization and improve bone formation in vivo.

关 键 词：组织工程 骨再生 骨髓基质细胞 细胞膜片 负压 

分 类 号：R783.1[医药卫生—口腔医学]