Cr^(3+)与EGCG共同作用对牙龈成纤维细胞67LR和EGFR表达的影响  

Complexation of Epigallocatechin-3-gallate with Cr^(3+) on the Expression of 67LR and EGFR on Gingival Fibroblast Cells

作　　者：方朱嫕 苏俭生 

机构地区：[1]同济大学口腔医院修复科 [2]同济大学口腔医学院口腔生物医学及转化医学实验室,上海200072

出　　处：《口腔颌面外科杂志》2013年第2期90-94,共5页Journal of Oral and Maxillofacial Surgery

基　　金：国家自然科学基金资助项目(30840031;30970726)

摘　　要：目的:分析表没食子儿茶素没食子酸酯(EGCG)与Cr3+共同作用,对牙龈成纤维细胞(HGF)的EGCG受体67LR和表皮生长因子受体(EGFR)的影响。方法:EGCG与Cr3+共同作用牙龈成纤维细胞24 h后,用Real-time PCR法检测mRNA表达,运用Western印迹法分析蛋白表达。结果:EGCG能抑制HGF细胞表面受体67LR mRNA以及蛋白水平的表达,加入Cr3+后减弱了EGCG对细胞67LR mRNA和蛋白的影响。EGCG作用24 h能降低EGFR mRNA和蛋白的表达,Cr3+(<200μmol/L)会减弱EGCG抑制EGFR的作用。结论:EGCG能降低HGF细胞67LR和EGFR的表达。加入Cr3+后对细胞67LR无明显影响,但降低细胞EGFR的表达。Objective： The purpose of this study was to examine the complexation effects of epigallocatechin-3-gallate （EGCG） with Cr3＋ on the expression of 67LR and EGFR on human gingival fibroblast cells. Methods： Real-time PCR was adopted to study the expression of 67LR, EGFR at mRNA levels and Western blot on protein levels. Results： EGCG suppressed the expression of 67LR, EGFR mRNA and proteins. The co-treatment of Cr3＋ and EGCG decreased EGCG- induced inhibition effect on 67LR expression. Cr3＋ in low concentration （lesser than 200 μmol/L） could decrease EGCG- induced inhibition effect on EGFR expression. Conclusion： EGCG inhibits the expression of 67LR and EGFR. EGCG mixed with Cr3＋ could reduce EGFR eexpression and has no significant effects on 67LR expression.

关 键 词：CR3+ 表没食子儿茶素没食子酸酯(EGCG) 层粘连蛋白受体(67LR) 表皮生长因子受体(EGFR) 

分 类 号：R780.2[医药卫生—口腔医学]