BABL/c小鼠感染大肠杆菌O127模型的建立及TGF-β1因子的荧光定量检测  

Establishment of a mouse model of Escherichia coli O127 infection and real-time PCR detection of TGF-β1 expression

作　　者：刘一[1,2] 隋丽华[2] 曾林[2] 宋宏立[3] 武际荣[4] 陈旖[2] 赵彦斌[2] 刘冰[2] 孙兆增[2] 王新[1] 

机构地区：[1]青岛农业大学动物科技学院,山东青岛266109 [2]军事医学科学院实验动物中心,北京100071 [3]青岛农业大学海都学院,山东莱阳265200 [4]青岛农业大学校医院,山东青岛266109

出　　处：《中国实验动物学报》2013年第2期13-16,共4页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金(编号:31272787;31071987)

摘　　要：目的建立BABL/c小鼠感染大肠杆菌O127的模型及TGF-β1的荧光定量PCR方法检测方法。方法通过灌胃的方式建立肠炎性小鼠模型,并利用嵌合荧光定量的方法对其结肠TGF-β1定量分析实验。结果成功建立了肠炎性小鼠模型,荧光定量PCR试验结果表明实验组TGF-β1表达量明显高于对照组。结论利用经口灌胃的方法可以成功的建立肠炎性小鼠动物模型,TGF-β1定量方法可以作为模型建立的评价标准。Objective To establish a mouse model of Escherichia coli O127 infection and to detect the TGF-β1 expression in the colon 0f mouse models by real-time PCR. Methods Thirty-six BALB/c mice （ male： female = 1 ： 1 ） were used in this study. Colon inflammation in the mice was induced by intragastric administration of Escherichia coil 0127. The expression of TGF-β1 in the colon tissue was analyzed using fluorescent quantitative PCR. Results The mouse models of colon inflammation were successfully established. The TGF-β1 expression level of the experimental group was sig- nificantly higher than that of the control group. Conclusions Mouse models of colon inflammation can be successfully es- tablished by intragastric gavage of E. coll. Quantitative PCR detection of the TGF-β1 expression can be used as a standard of evaluation of the establishment of the mouse models.

关 键 词：大肠杆菌O127 动物模型 荧光定量 TGF-Β1 

分 类 号：Q95-33[生物学—动物学]