DACH1、FASN和SREBP1在子宫内膜癌中的表达  

Expression of DACH1,fatty acid synthase and sterol regulatory element binding protein-1 in endometrial carcinoma

作　　者：刘洋[1] 李卫华[1] 吕清涛[2] 杨宁[1] 姜洁[1] 

机构地区：[1]山东大学齐鲁医院妇产科,济南250012 [2]山东中医药大学药学系,济南250355

出　　处：《山东大学学报（医学版）》2013年第4期71-76,共6页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金(81173614)

摘　　要：目的探讨细胞命运决定因子(DACH1)蛋白、脂肪酸合成酶(FASN)、固醇元件结合蛋白1(SREBP1)在子宫内膜癌中的表达及与临床病理参数的关系。方法应用免疫组织化学SP法检测DACH1、FASN、SREBP1在子宫内膜癌、不典型增生子宫内膜、复杂性子宫内膜、正常子宫内膜中的表达。结果 DACH1在子宫内膜癌中的表达显著低于非癌组,FASN及SREBP1在子宫内膜癌中的表达均显著高于非癌组。DACH1、FASN、SREBP1在子宫内膜癌中的表达率分别为40.0%、61.7%、65.0%,DACH1、FASN、SREBP1均与子宫内膜癌分化程度有关。DACH1与手术病理分期和病理类型有关,SREBP1与年龄有关。FASN、SREBP1与DACH1的表达呈负相关(r=-0.41,r=-0.40)。单因素分析结果显示,DACH1蛋白阳性组子宫内膜癌患者生存率高于DACH1蛋白阴性组(P<0.05);FASN及SREBP1蛋白阳性组子宫内膜癌患者生存率分别低于FASN蛋白及SREBP1蛋白阴性组(P<0.01,P<0.04)。结论 DACH1、FASN、SREBP1有可能成为子宫内膜癌判断预后的新指标。Objective To study the expression of dachshund （DACH1） protein, fatty acid synthase（FASN） and sterol regulatory element binding protein-1 （SREBP1） in endometrial carcinoma and the relation with clinical pathological parameters. Methods The immunohistochemistry SP method was used to examine the expression of DACH1, FASN and SREBP1 in endometrial carcinoma, endometrial atypical hyperplasias, complex endometrium and normal endometri- um. Resluts The positive rate of DACH1 in endometrial carcinoma was significantly less than that in benign groups, and the positive rates of FASN and SREBP1 in endometrial carcinoma were significantly higher than those in benign groups. The positive rates of DACH1, FASN, SREBP1 in endometrial carcinoma were 40.0% ,61.7% ,65.0%, and the DACH1, FASN, SREBP1 expressions were all significantly correlated with the differentiated degree. The DACH1 expression was significantly correlated with FIGO staging and histological types of endometrial carcinoma samples, and the SREBP1 expression was also significantly correlated with ages of the endometrial cancer patients. The DACH1 expression was negatively correlated with that of FASN and SREBP1, and the FASN expression was positively correla- ted with the SREBP1 expression. Single factor analysis showed that patients with positive DACH1 protein expression had a significant better overall survival time compared with patients with negative DACH1 protein expression （P 〈 0.05 ）. Single factor analysis showed that patients with positive FASN and SREBP1 protein expression had a significant poorer overall survival time compared with patients with negative FASN and SREBP1 protein expressions （ P 〈 0.05）. Conclusion DACH1, FASN and SREBP1 might be new markers for prognosis prediction of endometrial carcinoma.

关 键 词：子宫内膜肿瘤 DACH1蛋白 脂肪酸合成酶 SREBP1蛋白 免疫组织化学 

分 类 号：R737.33[医药卫生—肿瘤]