不同浓度纳米二氧化钛对大型溞的繁殖及富集和自净能力的影响  

Effects of different concentrations of TiO_2 nanoparticle on the reproduction, and bioaccumulation and depuration of Daphnia magna

作　　者：付保荣[1] 张楠[1] 武暕[1] 李楠[1] 张润洁[1] 惠秀娟[1] 李霞[1] 

机构地区：[1]辽宁大学环境学院,沈阳110036

出　　处：《生态科学》2012年第6期601-605,共5页Ecological Science

基　　金：国家水体污染控制与治理科技重大专项(2008ZX07526-006);辽宁省教育厅重点实验室项目(2009S044);辽宁省水环境生物监测与水生态安全重点实验室开放基金项目

摘　　要：为了弄清不同浓度条件下nTiO2对大型溞(Daphnia magna)生长繁殖的影响,以6个浓度nTiO2溶液进行大型溞21 d连续培养试验,结果表明:nTiO2浓度<1 mg L-1时,其对大型溞的生长繁殖的不利影响不显著;nTiO2浓度>1 mg L-1时,不利影响差异显著,且表现出随着浓度增加,存活率和繁殖受到明显抑制。第一次怀孕、产仔天数、产仔次数、一次产仔以及产仔总数都明显减小(p<0.05)。当nTiO2浓度为5 mg L-1时,繁殖基本上停止,内禀增长率rm由0.347(对照)降到0.106。nTiO2对大型溞的21 d LC50为4.16 mg L-1。24h富集试验结果表明:大型溞在24 h内对nTiO2的蓄积负荷量呈现随浓度和时间的增加而增强的趋势,在低浓度(2 mg L-1)和高浓度(10 mg L-1)条件下,大型溞均在24 h时达到最大负荷量。72 h净化试验结果是:大型溞对nTiO2的净化能力与其富集时的浓度条件有关,富集浓度低(2 mg L-1)的,其自身的净化能力强;富集浓度高(10 mg L-1)的,其自身的净化能力弱;前者的生物半减期T1/2为63.3 h,后者的生物半减期T1/2为181.9 h。We conducted a 21-day chronic test with six concentration in order to study the effects of different concentrations of TiO2 nanoparticle on the growth and reproduction of Daphnia magna.The results showed that at the concentration of TiO2< 1 mg L-1,adverse effects on the growth and reproduction of Daphnia magna were not significant.At the concentration of TiO2 >1 mg L-1,adverse effects were significant.As the concentration increased,the survival and reproduction were inhibited obviously.The days to first pregnancy,the days to first breeding,the number of broods per female,number of first broods per female,and the number of offsprings per female were all reduced significantly(p<0.05).At concentration of 5 mg L-1,breeding was basically stopped,and the intrinsic rate of natural increase(rm) was dropped from 0.347 to 0.106(control).The 21 d LC50was 4.16 mg L-1.The 24 h bioaccumulation experiment indicated that the greater the concentration and time,the higher the body burden.At both low concentration(2 mg L-1) and high concentration(10 mg L-1),the burden reached the maximum at 24 h.The 72 h depuration test suggested that the depuration was affected by the concentrations of accumulation.At low concentration(2 mg L-1),the depuration was stronger than at high concentration(10 mg L-1),and the biological halftime(T1/2) was 63.3 h and 181.9 h,respectively.

关 键 词：纳米二氧化钛 大型蚤 繁殖 富集 净化 

分 类 号：X174[环境科学与工程—环境科学]