TLR9多态性和IFN-γ表达与结直肠癌的相关性  

Association between Toll-like Receptor 9 Polymorphisms and IFN-γ Expression with Colorectal Carcinoma

作　　者：李世宁[1] 戴穹[2] 杨志惠[1] 

机构地区：[1]泸州医学院病理学教研室,泸州646000 [2]泸州医学院解剖学教研室,泸州646000

出　　处：《四川解剖学杂志》2012年第4期12-15,共4页Sichuan Journal of Anatomy

基　　金：国家自然科学基金资助项目(编号:81101679)

摘　　要：目的探讨Toll样受体9(Toll-like receptor 9,TLR9)-1237T/C、-1486T/C和2848G/A多态性和IFN-γ表达与四川结直肠癌相关性。方法 246名结直肠癌患者和246名健康对照个体TLR9多态性位点基因型采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)分析,ELISA方法检测结直肠癌组织匀浆中IFN-γ、TGF-β和TNF-α细胞因子蛋白水平。结果 TLR9-1237T/C和2848G/A多态性基因型在病例-对照组中频率无显著性差异,-1486T/C CC基因型显著增加了个体患结直肠癌的风险;IFN-γ在-1486T/C CC基因型个体结直肠癌组织中浓度显著降低,在-1486T/C不同基因型个体组中的表达有显著性差异。结论 TLR9-1486T/C可调节IFN-γ在结直肠癌组织中的表达,与结直肠癌发生相关。Objective To investigate association between TLR9-1237T/C,-1486T/C and 2848 G /A polymorphisms and colorectal carcinoma(CRC),and to explore effect of polymorphisms on cytokines in the CRC biopsies.Methods The genotypes of these three loci among 246 patients with CRC and 246 healthy controls were determined by polymerase chain reaction-restriction fragment lengthy polymorphism(PCR-RFLP).Cytokines in CRC biopsies homogenate were measured by Enzyme-linked immunosorbent assay(ELISA).Results There were no significant difference of TLR9-1237T/C and 2848 G/A genotypes among the patient and the control.However,-1486T/C CC genotype significantly increased the CRC risk as compared with-1486T/C TT one.Protein level of IFN-γ was significantly elevated in patients with-1486T/C TT genotype.Conclusion These data suggested that TLR9-1486T/C was related IFN-γ expression,and they both were associated with CRC.

关 键 词：TOLL样受体9 结直肠癌 多态性 细胞因子 

分 类 号：R735.3[医药卫生—肿瘤]