膜联蛋白A2在乳腺癌及乳腺纤维腺瘤中的表达及意义  

Annexin A2 was Differentially Expressed Among Beast Fibroadenoma and Different Stages of Breast Cancer Tissues

作　　者：李金穗[1,2] 赵锐 幸天勇 李敬东 陈果[4] 杨正伟[1] 敬保迁[4] 邓世山[1,5] 

机构地区：[1]川北医学院形态学研究所,南充637000 [2]川北医附院普外三科,南充637000 [3]川北医附院耳鼻咽喉头颈外科,南充637000 [4]川北医学院分子生物所,南充637000 [5]川北医学院人体解剖学教研室,南充637000

出　　处：《四川解剖学杂志》2012年第4期33-36,共4页Sichuan Journal of Anatomy

基　　金：国家自然科学基金面上项目(项目批准号:81172496);四川省科技支撑计划项目(课题编号:2009SZ0116)

摘　　要：目的研究膜联蛋白A2(Annexin II,ANX A2)在乳腺浸润性导管癌和乳腺纤维腺瘤中表达,以探讨其在乳腺癌发生发展中的作用。方法分别使用免疫组织化学染色(SP)法和逆转录聚合酶链反应(RT-PCR)法检测乳腺浸润性导管癌和乳腺纤维腺瘤中ANX A2的蛋白表达水平和mRNA表达水平,并分析其在不同病理分级、临床分期及有无淋巴结转移的乳腺浸润性导管癌组织中的表达情况。结果免疫组化结果显示乳腺浸润性导管癌组织中ANX A2表达高于乳腺纤维腺瘤(P<0.05),RT-PCR结果也显示乳腺浸润性导管癌中ANX A2mRNA的表达高于乳腺纤维腺瘤(P<0.05)。乳腺癌中ANX A2的表达与年龄、病理分级、临床分期和淋巴结转移呈正相关。结论乳腺浸润性导管癌中annexinA2表达明显高于乳腺纤维腺瘤,annexinA2可能是乳腺癌的生物学标记物之一,其在乳腺癌的发生发展中可能扮演重要的角色。Objective To research that annexinA2 may play a role in breast cancer by observing expression of annexinA2 in both human breast cancer and fibroadenoma. Methods Different pathological gradings, clinical stagings and lymph node metastasis or not of breast cancer tissues, fibroadenoma tissues control being used,were observed by immunohistochemical staining and RT-PCR. Results Expession of protein and gene of annexinA2 in breast cancer tissues were higher than in breast fibroadenoma tissues （P〈0.05）. Expression of annexinA2 was positively correlao ted with age, pathological gradings, clinical stagings and lymph node metastasis in breast cancer. Conclusion Expression of annexinA2 was up-regulated in breast cancer cells,indicating that annexinA2 may be a biological marker and play an important role in breast cancer.

关 键 词：乳腺浸润性导管癌 膜联蛋白A2 免疫组化染色法 逆转录聚合酶链反应法 

分 类 号：R737.9[医药卫生—肿瘤]