肝癌组织中多基因甲基化与乙型肝炎病毒感染的关系  

The relationgship between Hepatitis B Virus infection and Multigene methylation in hepatocellular carcinoma

作　　者：张吉才[1] 余宗涛[1] 吕军[1] 谢飞[1] 高波[1] 李海平[1] 

机构地区：[1]湖北医药学院附属太和医院检验部,湖北十堰442000

出　　处：《临床内科杂志》2013年第3期163-165,共3页Journal of Clinical Internal Medicine

基　　金：湖北省教育厅重大资助项目（Z20082401）

摘　　要：目的 评估HBV感染与非HBV感染的肝癌组织中多基因（p14、p15、p16和RB）甲基化状态,探讨HBV感染诱导基因甲基化在肝癌发病机制中的作用.方法 运用甲基化特异性聚合酶链反应（PCR）方法检测32例HBV感染和12例非HBV感染患者的肝癌组织中p14、p15、p16和RB的甲基化状态,应用实时荧光定量PCR技术检测44例血标本中HBV DNA.结果 44例肝癌组织中p14、p15、p16和RB基因的甲基化检出率分别为34.1％、56.8％、70.5％和27.3％；HBV感染与非HBV感染患者的肝癌组织中p14、p15、p16和RB4种基因的甲基化检出率分别为43.8％和8.3％、68.9％和25.0％、90.6％和16.7％、28.1％和25.0％；HBV感染与p14、p15、p16基因的甲基化明显相关（P=0.048、0.035、0.020）,HBV DNA扩增与p14、p15、p16基因的甲基化明显相关（P均＜0.05）.结论 （1）p14、p15、p16、RB基因甲基化是肝癌发生的频发事件；（2）HBV病毒感染与p14、p15、p16基因的甲基化相关；（3）HBV感染诱导相关基因高甲基化可能是HBV相关性肝癌的发病机制之一.Objective To assess the methylation status of（pl4,pl5,pl6,RB） in HCC with or without HBV infection, and discuss the possible role of HBV-induced hypermethylation in the mechanism of hepatocarcinogenesis. Methods Methylation status of p14,p15,p16,RB in 32 case of HCC with HBV infection and 12 cases of HCC without HBV infection were detected by methylation-specific polymerase chain reaction and HBV-DNA of the plasma were detected by quantitative real-time polymerase chain reaction. Results Methylation frequencies of them between HCC with HBV infection and HCC without HBV infection were 43.8% vs 8.3% （p14） ,68.9% vs 25.0% （p15） ,90.6% vs 16.7% （p16） and 28. 1% vs 25.0% （ RB）, respectively; HBV infection was associated with p14, p15, p16, RB gene methyl- ation（ P = 0. 048,0. 035,0. 020）, HBV-DNA replication was associated with p14, p15, p16, RB gene methylation（P = 0. 048,0. 035,0. 020）. Conclusion （ 1 ） p14, p15, p16, RB gene methylation is a frequent epigenetic event in the tumor development ; （ 2 ） HBV infection is associated with p14, p15, p16, RB gene methylation ; （3）High methylation rate of p14 ,p15, p16 in HCC with HBV infection suggests that HBV- induced hypermethylation may be one of the mechanisms of HBV related hepatocellular carcinogenesis.

关 键 词：肝癌 甲基化 甲基化特异性聚合酶链反应 HBV病毒 感染 

分 类 号：R734.2[医药卫生—肿瘤]