δ-catenin促进卵巢癌细胞的增殖和侵袭  

δ-catenin Promote the Proliferation and Invasion of Ovarian Cancer Cells

作　　者：方媛媛[1] 杜强[1] 王秀霞[1] 张淑兰[1] 

机构地区：[1]中国医科大学附属盛京医院妇产科,沈阳110004

出　　处：《中国医科大学学报》2013年第4期334-338,共5页Journal of China Medical University

摘　　要：目的探讨δ-catenin在卵巢癌中的生物学作用。方法在SKOV3和SW626卵巢癌细胞系中通过siRNA干扰降低δ-catenin表达，在OVCAR3细胞中转染δ-catenin增加δ-catenin的表达；通过MTT、集落形成实验、软琼脂集落形成实验和基质胶侵袭实验，检测δ-catenin对卵巢癌细胞的增殖和侵袭的作用；通过流式细胞仪检测细胞周期的变化。结果在SKOV3和SW626卵巢癌细胞系中敲减δ-catenin后细胞增殖能力和侵袭能力明显降低，同时在OVCAR3细胞中转染δ-catenin后细胞增殖能力和侵袭能力显著提高；δ-catenin可以通过cyclinD1促进GrS转换，调节细胞周期进展，进而促进卵巢癌细胞的生长。结论δ-catenin为研究卵巢癌的生物学作用提供了一个好的开端，可能成为卵巢癌治疗中的一个新的靶点。Objective To explore the biological effect of δ-catenin to ovarian cancers. Methods Decrease the expression of δ-catenin in SKOV3 and SW626 cell lines by siRNA transfecfion and increase the expression of δ-catenin in OVCAR3 cell lines by plasmid lxansfection. Examine the proliferation and invasion effect of δ-catenin to ovarian cancers cells by MTT, colony formation assay, soft agar colony formation assay and matrigel invasion assay. Examine cell cycle change by flow cytometer. Results Cell proliferation ability and invasion ability decreased obviously after δ-catenin knockdown in SKOV3 and SW626 cell lines, while increased obviously after δ-catenin transfection in OVCAR3 cell lines.δ-catenin can regulate cell cycle progress and cancer cell growth by cyclin D1 which can promote G1-S shift. Conclusion δ-catenin might serve as a molecular target closely associated with biological effect for future development of ovarian cancer thempeutics.

关 键 词：卵巢癌 δ-catenin 增殖 侵袭 细胞周期 

分 类 号：R737.31[医药卫生—肿瘤]