“西南302”油菜(Brassica napus)种子贮藏蛋白的分离及部分性质  

Extraction and characteristics of storage proteins in rapeseed (Brassica napus L.)

作　　者：文建军[1] 何应森[1] 何荻平[1] 刘世贵[1] 高荣[1] 

机构地区：[1]四川大学生命科学院,中国成都610064

出　　处：《西南农业学报》2000年第2期46-50,共5页Southwest China Journal of Agricultural Sciences

摘　　要：用油菜品种“西南３０２”（Ｂ．ｎａｐｅｓ）为材料，研究了油菜种子贮藏蛋白的分离及部分性质。用 Ｎａ２ＨＰＯ４－ＮａＯＨ（ｐＨ １１．４）缓冲液抽提蛋白。 ４％～２０％连续梯度ＳＤＳ—ＰＡＧＥ垂直板电泳表明，该贮藏蛋白组分十分复杂，其分子量范围是 ８， ０００～ １９８，０００，带型不仅多，而且含量很不一致，其中低分子量蛋白质（８， ０００～ ３０， ０００）含量占 ５０％～ ６０％以上。凝胶等电聚焦电泳表明，等电点范围为 ｐＨ３． ５～ ９，其中酸性蛋白质含量占 ６５％左右。In this paper, the extraction and characteristics of storsge proteins of 'South west 302' rapeseed(B. napus) were studied systematically using Na2HPO4-NaOH buffer (pH 11.4) to extract proteins, three key problems to study plant seed proteins were solved. The results of 4% - 20% gradient PAGE on vertical plate showed that the compositions of rapeseed storage protein were complicate. Molecular weight (MW) of the proteins were between 8.000~198,000. There were 19 bands on the SDS-PAGE and their contents were different significantly. The contents of lower MW proteins of 8,000-30,000 were up to 60%, Gel isoelectrofocusing electrophoresis (IEF) suggested that the pI of these proteins was between pH 3.5-9.0. Among them, acidic proteins were 65% of total protein.

关 键 词：油菜种子 贮藏蛋白质 反抽提法 SDS-PAGE IEF 

分 类 号：S565.401[农业科学—作物学]