维吾尔族和汉族不同龋敏感儿童远缘链球菌的荧光定量PCR检测  

The detection of Streptococcus sobrinus in different caries-sensitive of Uyghur and Han children by Fluorescence quantitative polymerase chain reaction

作　　者：刘芳[1,2] 冯锦虹[1,2] 仵楠[1,2,3] 赵今[1] 

机构地区：[1]新疆医科大学第一附属医院牙体牙髓科 [2]新疆维吾尔自治区口腔医学研究所,乌鲁木齐830054 [3]新疆石河子大学医学院第一附属医院口腔科,新疆石河子832000

出　　处：《新疆医科大学学报》2013年第4期494-499,共6页Journal of Xinjiang Medical University

基　　金：新疆医科大学第一附属医院博士后后续基金(59029)

摘　　要：目的研究维吾尔族和汉族不同龋敏感儿童牙菌斑中远缘链球菌的检出量,分析远缘链球菌与不同民族儿童乳牙龋齿发生的关系,为维吾尔族和汉族儿童乳牙龋的防治提供依据。方法采用T-A克隆技术制备远缘链球菌ATCC6715、内参基因16SrRNA的质粒标准品,应用SYBR Green I荧光定量聚合酶链反应对3～5岁维吾尔族和汉族不同龋敏感儿童牙菌斑中远缘链球菌进行绝对定量检测,以龋失补牙数≥5颗为高龋组和龋失补牙数=0颗为无龋组,使用SPSS17.0软件包对结果进行析因分析。结果维吾尔族儿童高龋组远缘链球菌的检出量为(1.06×105±4.27×103)copies/μL,高于无龋组[(2.55×104±2.84×103)copies/μL],差异有统计学意义(P<0.05);汉族儿童高龋组远缘链球菌的检出量为(8.29×104±1.20×104)copies/μL,高于无龋组[(1.54×103±9.74×102)copies/μL],差异有统计学意义(P<0.05),同时维吾尔族儿童远缘链球菌的检出量高于汉族儿童,差异有统计学意义(P<0.05)。结论远缘链球菌可能是维吾尔族和汉族儿童乳牙龋高发的危险因素,维吾尔族远缘链球菌高检出量可能与不同生活习俗和饮食习惯有关。Objective To detect the presence of Streptococcus sobrinus in dental plaque of children from Urumqi and correlate it to dental caries, and to assess their association with the prevalence of deciduous caries. Methods Using the T-A cloning method to construct the standards of S. sobrinus ATCC6715 and Uni for real-time fluorescence quantitative polymerase ed, 70 of which were Uyghur, between 3 and 5 years＇ chain reaction, sample of 169 children were collect old. Dental plaque samples were collected and bacte rial DNA extracted,which subjected to FQ-PCR analysis. The data were statistically analyzed by SPSS 17.0 software package. Results The quantity of S. sobrinus in plaque of Uyghur children with high dmft was higher than that in caries-free children （S. sobrinus .. 1.06× 105 ±4.27 × 103 vs 2.55 × 104 ±2.84 ×103 , P 〈0.05）, while there was statistically significant difference of HAN peo ×104 vs 1.54×10：3±9.74×102, P〈0.05）. Conclusion These results ple （S. sobrinus. 8.29× 104 ＋1.20 indicate that Uyghur and Chinese preschool children harboring S. sobrinus have a significantly high incidence of dental caries

关 键 词：链球菌 远缘 荧光定量聚合酶链反应 维吾尔族儿童 汉族儿童 

分 类 号：R781.1[医药卫生—口腔医学]