HPV58型在粤东地区宫颈癌中的病毒含量和感染状态研究  

Viral Load and Infection Status of HPV Type 58 in Cervical Cancer in Eastern of Guangdong Province

作　　者：周莉[1] 魏晓龙[1] 黄萍[1] 关明飞[1] 

机构地区：[1]汕头大学医学院附属肿瘤医院,广东汕头515031

出　　处：《肿瘤学杂志》2013年第4期268-272,共5页Journal of Chinese Oncology

基　　金：广东省汕头市科技计划项目(汕府科201146号)

摘　　要：[目的]建立灵敏而稳定的人乳头状瘤病毒(human papillomavirus,HPV)58型病毒含量与整合状态检测方法,并探讨HPV58型在我国粤东地区妇女宫颈癌中的感染特征。[方法]PCR扩增HPV58E2和E6基因,PCR产物纯化,连接入T载体,构建标准品;检测30例宫颈癌和40例对照组织样本中HPV58的病毒含量和整合状态。[结果]成功构建含有HPV58E2或E6片段的T载体质粒作为检测HPV58型的病毒含量和判断感染状态标准品。HPV58型在宫颈癌中的感染率和病毒含量较正常宫颈组织均高,并且HPV58在粤东地区宫颈癌中的感染状态以整合型(66.67%)和混合型(33.33%)为主,和正常宫颈组织中感染状态分布有显著差异。[结论]采用荧光定量PCR法所建立的HPV58型病毒含量和整合状态的检测方法具有灵敏性和稳定性,HPV58型在粤东地区宫颈癌中的感染以整合状态为主。[Purpose]To establish a sensitive and stable method for measuring human papilloma virus （HPV） type 58 infection status of cervical cancer patients in eastern region of Guangdong province. [Methods] HPV58E2/E6 genes were amplified, PCR products were purified and linked to T vector,and constructed the standard plasmid. HPV58 viral load and infection status of 30 cervical cancer patients and 40 controls were detected. [Resultsl The standard plasmid of real- time fluorescence quantitative PCR for detecting HPV58 E2/E6 fragments was stable and sen- sitive. HPV58 infection rate and viral load in cervical carcinoma were higher than those of con- trols. Further, HPV58 infection status were integrated （66.67%） and mixed status （33.33%） in cervical cancer,which were different from controls. [Conclusion ] The standard plasmid of fluo- rescence quantitative PCR is a high sensitivity and stability method to detect HPV58 virus load and infection status,and integration status is the main infection status of HPV58 in the cervical cancer patients in eastern region of Guangdong province.

关 键 词：人乳头状瘤病毒 宫颈肿瘤 病毒含量 感染 广东 

分 类 号：R737.33[医药卫生—肿瘤] R730.231.3[医药卫生—临床医学]