辐射诱发白血病初期过程的细胞和分子遗传学研究  

CYTOGENETIC AND MOLECULAR BIOLOGICAL STUDIES ON RADIATION INDUCED LEUKEMIA DURING EARLY PERIODS

作　　者：陈英[1] 段志凯[2] 徐洪兰[1] 王仲文[1] 张淑贤[1] 

机构地区：[1]中国辐射防护研究院 [2]中国辐射防护研究院1太原030006

出　　处：《辐射研究与辐射工艺学报》2000年第1期1-6,共6页Journal of Radiation Research and Radiation Processing

基　　金：核工业科学基金!J94Q61057

摘　　要：以小鼠为实验模型，通过对经Ｘ射线照射后 １２～３２ｄ的小鼠染色体Ｇ带核型分析，发现与白血病发生有密切关系的特异性染色体畸变是１５号染色体三体和 １２号染色体Ｅ－Ｆ区的易位。在细胞遗传学分析基础上，用 ＰＣＲ－ＳＳＣＰ方法检测了与特异性染色体畸变有关的受照射小鼠ｃ－Ｉｎｙｃ原癌基因和ｐ(５３)抑癌基因的结构变化。选用ｃ－ｍｙｃ基因ＰＣＲ－ＳＳＣＰ试剂盒对正常和受照射小鼠进行了分析，实验结果未发现特异性改变。用 ＰＣＲ－ＳＳＣＰ和 Ｓｏｕｔｈｅｒｎ Ｂｌｏｔ方法又分析了部分胸腺瘤小鼠的ｐ(５３)基因变化，结果发现，个别样品中在第５、７、８对外显子上有点突变发生，但未发现有相同的 ｐ(５３)基因改变．It was detected by chromosome G-band analysis of experimental mice after 12-32 days of x-ray irradiation that the characteristic chromosome aberrations of trisomy 15 and translocation in E--F regions of chromosome 12 were related closely to leukemia generation. Based on the cytogenetic analysis, tile structural changes of c-myc protooncogene and p53 anti-oncogene were examined by PCR-SSCP and Southern Blot methods. The significant mutations have hot been found by the examination of c-myc gene in thymocytes of normal and irradiated mice. The analysis resuts of p53 gene in several thymoma mice showed that there were not common p53 gene mutations, except a few samples with point mutations on the exon 5, 7 or 8.

关 键 词：辐射 白血病 细胞遗传学 分子遗传学 

分 类 号：R733.7[医药卫生—肿瘤]