大豆异黄酮抗高尿酸血症活性研究初探  

Study on Anti-hyperuricemia Activity of Soybean Isoflavone

作　　者：陈玉胜[1] 

机构地区：[1]南京晓庄学院生物化工与环境工程学院,江苏南京211171

出　　处：《大豆科学》2013年第2期279-282,共4页Soybean Science

基　　金：江苏省高等学校大学生实践创新项目(2160081)

摘　　要：为研制新型无副作用的降血尿酸药物,在氧嗪酸钾盐诱导小鼠产生高尿酸血症模型的基础上,探讨大豆异黄酮的抗高尿酸活性。将昆明雄性小鼠随机分为6组,即正常对照组、高尿酸血症模型对照组、模型给药组(分为大豆异黄酮25,50和100 mg·kg-1剂量组、别嘌醇5 mg·kg-1剂量组)。连续7 d每天灌胃氧嗪酸钾盐(250 mg·kg-1)1 h后灌胃给药。分别测定血清尿酸、肌酐与尿素氮水平及尿液尿酸和肌酐水平。结果表明:与高尿酸血症模型组相比,大豆异黄酮显著或极显著降低高尿酸血症小鼠血清尿酸、肌酐和尿素氮水平,提高24 h尿液尿酸和肌酐排泄量以及尿酸排泄分数。综合考虑,大豆异黄酮通过促进肾脏尿酸排泄作用,从而体现抗高尿酸活性。The anti-hyperuricemia activity of soybean isoflavone in hyperuricemic mice induced by potassium oxonate was dis- cussed. Kunming male mice were divided randomly into normal control group, and 5 hyperuricemic groups with model control, 5 mg· kg-1 allopurinol and 25,50, 100 mg· kg-1 soybean isoflavone. Mice were orally administered once daily with 250 mg·kg- 1 potassium oxonate for 7 continuous days to create the model. Three doses of soybean isoflavone were orally initia- ted on the day 1 h after potassium oxonate was given, separately. Serum uric acid, creatinine and urea nitrogon levels, as well as urinary uric acid and creatinine levels were measured. The results indicated that, compared with model group, soybean isofla- vone significantly reduced serum uric acid,creatinine and urea nitrogon levels,increased 24 h uric acid and creatinine excre- tion, and fractional excretion of uric acid in hyperuricemic mice. In conclusion, the anti-hyperuricemia effect of soybean isofla- vone was attributed to uric acid excretion enhancement.

关 键 词：大豆异黄酮 高尿酸血症 氧嗪酸钾盐 小鼠 

分 类 号：S565.1[农业科学—作物学]