苦参碱对前列腺癌PC-3M细胞生长抑制和凋亡的影响  

Matrine Induced Human Prostate Cancer Pc-3m Cell Apoptosis and Groth Inhibition

作　　者：金光虎[1] 夏海波[1] 李海峰[1] 张伟[1] 崔向宇[1] 

机构地区：[1]赤峰学院附属医院泌尿外科,内蒙古赤峰024000

出　　处：《内蒙古医学杂志》2013年第1期1-5,共5页Inner Mongolia Medical Journal

摘　　要：目的:探讨苦参碱治疗前列腺癌的可行性及机制。方法:用不同药物浓度苦参碱诱导PC-3M细胞不同时间后,在相差倒置显微镜观察细胞形态;用MTT法测细胞的增殖抑制情况;用流式细胞仪(FCM)进行细胞凋亡率检测;RT-PCR法检测PC-3M细胞内bcl-2、bax基因表达水平。结果:用苦参碱干预后随着药物浓度增加PC-3M细胞株生长明显受抑制;在FCM上可见凋亡率逐渐增加;PC-3M细胞中bcl-2 mRNA表达水平逐渐下调,bax mRNA表达水平逐渐上调,且呈时间-浓度依赖性。结论:苦参碱体外能有效抑制前列腺癌细胞株生长,其机制可能与诱导细胞凋亡、下调bcl-2表达及上调bax水平有关。Objective： To study the feasibility of treatment of prostate cancer using matrine and its mecha- nism. Methods： Add a certain concentration of matrine in human prostate cancer pc- 3m cell incubated together, using the method of MTT and flow cytometry to detect the changes in cell proliferation and apoptosis, using re- verse transcription polymerase chain reaction（RT- PCR） to detect the expression level of bcl- 2 and bax gene in human prostate cancer pc - 3m cell. Result： matrine can inhibit the growth of human prostate cancer pc - 3m cell obviously, and showing a time concentration - dependent; The rate of apoptosis increases in flow cytometry; bcl- 2 mRNA expression level gradually lowered and bax mRNA expression levels gradually raised. Conclusion：ma- trine can inhibit the growth of human prostate cancer pc - 3m cell in vitro tests, the mechanism may relate to in- duced apoptosis and down to bcl - 2 expression and up - regulation of bax level.

关 键 词：苦参碱 前列腺癌 细胞凋亡 

分 类 号：R735.7[医药卫生—肿瘤]