三清降糖方的药效物质基础研究  

Pharmacodynamic Material Basis of Sanqing Jiangtang Decoction

作　　者：吕立勋[1] 李唐棣[1] 王旭[1] 吴范武[2] 赵翠[2] 

机构地区：[1]河北联合大学药学院,河北唐山063000 [2]河北联合大学中医学院,河北唐山063000

出　　处：《中国实验方剂学杂志》2013年第9期267-270,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：2011年唐山市科技支撑项目(1113020112a)

摘　　要：目的:研究三清降糖方及各分离化学部位的对α-葡萄糖苷酶的抑制作用,对效应显著部位进行物质基础定性分析。方法:大孔吸附树脂分离制备各化学部位样品(JT-1～JT-13);葡萄糖氧化酶法和淀粉耐量实验考察样品对α-葡萄糖苷酶活性的影响。结果:JT-1,JT-3,JT-6,JT-7部位对α-葡萄糖苷酶有显著抑制作用,其中JT-7部位对α-葡萄糖苷酶的抑制作用最显著。结论:生物碱和苷类成分可能是三清降糖方对α-葡萄糖苷酶的抑制作用的主要物质基础。Objective：To evaluate the inhibitory activity of Sanqing Jiangtang decoction and chemical constituents on alpha glucosidase enzyme and to exploit the bioactive constituents.Method： The samples were prepared by macroporous adsorptive resins（JT-1-JT-13）.Glucose oxidase method and starch tolerance test experiments were adopted to evaluate the inhibitory activity of Sanqing Jiangtang decoction and its different separated fractions.Result： It was found that fractions（JT-1,JT-3,JT-6,JT-7）,separated from Sanqing Jiangtang decoction,mainly contributed to the inhibitory activity.JT-7 showed the most significant activities in the experiments.Conclusion：Glycosides and alkaloid may be the principle effective substance in the active fraction.

关 键 词：三清降糖方 Α-葡萄糖苷酶 物质基础 

分 类 号：R284.1[医药卫生—中药学]