不同宫颈病变患者血液中Bcl-2、P53和Caspase-3的表达及其相互关系  

The expression and their mutual relations of Bcl-2,P53 and Caspase-3 in the blood of patients with cervical lesions

作　　者：白雪[1] 黄可欣[2] 石搏[2] 李锐[3] 黄可敬[4] 

机构地区：[1]中国人民解放军第二零二医院,辽宁沈阳110003 [2]吉林大学基础医学院形态学中心 [3]吉林省人民医院病理科 [4]吉林省长春市中心血站

出　　处：《中国妇幼保健》2013年第13期2144-2146,共3页Maternal and Child Health Care of China

基　　金：吉林省教育厅基金项目〔2012460〕

摘　　要：目的:探讨Bcl-2、P53和Caspase-3在不同宫颈病变发生、发展中的作用及其相互关系。方法:对37例宫颈炎、39例宫颈内瘤变、24例宫颈癌患者抽取血液,采用Elisa方法检测血清中Bcl-2、P53和Caspase-3蛋白的含量,采用流式细胞术检测血细胞中Bcl-2、P53和Caspase-3的阳性率,结合临床病理特点分析。结果:宫颈炎患者血清中Bcl-2、P53蛋白含量及血细胞中Bcl-2、P53阳性率明显低于宫颈内瘤变和宫颈癌患者(P<0.01),且宫颈内瘤变患者明显低于宫颈癌患者(P<0.05);宫颈炎患者血清中Caspase-3蛋白含量及血细胞中Caspase-3的阳性率明显高于宫颈内瘤变和宫颈癌患者(P<0.01),且宫颈内瘤变患者明显高于宫颈癌患者(P<0.05)。结论:Bcl-2、P53和Caspase-3蛋白在宫颈癌发生、发展过程中发挥着即独立又协同的作用。Objective： To explore their mutual relationship of Bcl -2, P53 and Caspase - 3 in different cervical lesions, the role in the development and occurring of cervical lesions, by observing the expression of Bcl - 2, P53 and Caspase - 3 in the blood. Methods： Blood of 100 patients with high -risk （37 cases of cervicitis, 39 cases of cervical intraepithelial neoplasia, 24 cases of cervical cancer） was taken. ELISA was used to detect serum Bcl- 2, P53 and Caspase -3 protein content. Flow cytometry was used to detect Bcl -2, P53 and Caspase -3 positive rates in blood cells. Clinical and pathological features were analyzed. Results： Serum Bcl -2 and P53 protein contents and the Bcl - 2, I＇53 positive rates in blood ceils of eervicitis patients were significantly lower than those of patients with cervical intraepithe- lial neoplasia and cervical cancer （ P 〈 0. 01 ） and the above indexes of cervix neoplasia patients were significantly lower than those of patients with cervical cancer （P 〈 0.05） . Serum Caspase - 3 protein content and positive rate in the blood ceils of cervicitis patients were signifi- cantly higher than those of patients with cervical intraepithelial neoplasia and cervical cancer （ P 〈0. O1 ） and the above indexes of cervix ne- oplasia were significantly higher than those of cervical cancer patients （ P 〈 0. 05） . Conclusion： Bcl - 2, 1：＇53 and Caspase - 3 protein play an independent but synergistic role in development and process of cervical cancer.

关 键 词：宫颈癌宫颈癌前病变Bcl-2 P53 CASPASE-3 

分 类 号：R737.33[医药卫生—肿瘤]