复合木质纤维素酶菌株筛选及其培养条件优化  

Isolation of Strain Producing Complex Lignocellulase and the Optimization of Culture Conditions

作　　者：于俊杰[1,2] 赫荣琳 武改红 张粲[1,3] 陈树林 张同存[1] 

机构地区：[1]天津科技大学生物工程学院,天津300457 [2]天津工业生物技术研究所天津市工业生物系统与过程工程重点实验室,天津300308 [3]天津工业生物技术研究所 天津市工业生物系统与过程工程重点实验室,天津300308

出　　处：《生物技术通报》2013年第4期101-109,共9页Biotechnology Bulletin

基　　金：国家“863”计划项目(2012AA101807,2012AA023202)

摘　　要：通过富集培养、平板初筛、平板点接,从土壤中筛选得到一批产木质纤维素酶系较全的菌株,经摇瓶复筛验证确定26-6为出发菌株。该菌经PDA平板菌落形态观察、孢子扫描电镜观察以及ITS序列片段分析,初步鉴定该菌为草酸青霉组菌株,并命名为Penicillium oxalicum EH26-6。随后对菌株的摇瓶发酵条件进行研究,最终确定了最佳产酶条件:玉米芯为碳源,玉米浆干粉为有机氮源,初始pH5.0,温度30℃,接种量10%。本研究为低成本生产复合纤维素酶系的生产提供一个备选菌株。Based on enrichment culture, initial screening, and further screening by spotting vaccination, a group of degradation lignocelluloses strains were isolated from soil. Then strains were cultured in the flask to develop the repeated screening, and enzymes activity was tested, finally a filamentous fungus 26-6 was screened. Then colonial morphology of the strain on PDA plate was observed, spore morphology was also observed by scanning electron microscope. According to the ITS rDNA sequence analysis, the fungi was initially identified as a Penicillium oxalicum strain and named Penicillium oxalicum EH26-6. Culture optimization was then carried out in flask scale and finally the optimal culture condition was determined as corn cob for carbon source, corn steep powder for organic nitrogen source, pH5.0, temperature 30℃, inoculation 10%. The research provided an alternative strain for the low cost production of composite cellulose enzyme.

关 键 词：筛选 木质纤维素酶 培养条件 优化 

分 类 号：TQ925[轻工技术与工程—发酵工程]