中药复方“松友饮”体内药效物质基础研究  

Preliminary Research on Effective Substances of Chinese Herbal Compound Songyou Yin in Vivo

作　　者：黄修燕[1] 黄自丽[2] 郑媛婷[3] 郑起[1] 黄新余[1] 

机构地区：[1]上海交通大学附属第六人民医院,上海200233 [2]上海市徐汇区中心医院,上海200031 [3]上海复旦大学药学院,上海201203

出　　处：《中华中医药学刊》2013年第5期979-983,共5页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(81272401);上海市卫生局中医药科研基金资助项目(2010L059A);上海市卫生局中医药科研基金资助项目(2012QJ001A)

摘　　要：目的:研究中药复方"松友饮"的体内药效物质基础。方法:以MHCC97H细胞构建裸鼠原位移植瘤模型,12只成模裸鼠随机平均分成对照组和"松友饮"组,肝脏接种肿瘤24 h内开始1次/d灌胃给药。在第7次灌胃结束后2 h,脱颈处死裸鼠收集血样。应用ABI 4000 Q-TRAP仪器和TIC总离子扫描方式进一步研究、分析含药血清的药效物质基础。结果:成功构建裸鼠原位移植瘤模型,获取有效的含药血清。发现体内有两个主要的吸收入血峰,分别为RT=29.61,m/z为213.3;RT=53.69,m/z为523.8。通过比对发现"松友饮"吸收入血峰来自组方药材成分。结论:给药7 d的含药血清药效物质基础主要为吸收入血的丹参酮IIA和黄芪甲苷,其分子基础尚需进一步研究。Objective：To investigate the in vivo effective substances of Chinese herbal compound Songyou Yin.Methods：Metastatic human HCC nude mice models were established by orthotopic implantation with human HCC cell line MHCC97H.Twelve nude mice bearing orthotopic xenografts were randomized into control group and Songyou Yin group（6 in each group）.Daily intragastric administration of Songyou Yin was given 24 hours after orthotopic implantation.Two hours after the 7th intragastric administration,mice were sacrificed by cervical dislocation to collect serum samples,which were determined by ABI 4000 Q-TRAP for further analyses of effective substances of Songyou Yin.Results：Metastatic human HCC nude mice models were successfully established,and medicated serum was collected.The two predominant peaks of Songyou Yin derived from those of components were found,which were RT=29.61,m/z=213.3,and RT=53.69,m/z=523.8,respectively.Conclusion：The effective substances of medicated serum of Songyou Yin after the 7th intragastric administration were tanshinone IIA and Astragaloside IV,whose detailed molecular basis remains to be elucidated.

关 键 词：中药复方 松友饮 药效物质基础 

分 类 号：R222.19[医药卫生—中医基础理论]