黄芪多糖对人胃癌细胞系MGC-803的生长抑制作用及细胞周期的影响  

Effect of Astragalus Polysaccharides on Proliferation and Cell Cycle of Human Gastric Carcinoma Cell Line MGC-803

作　　者：周杰[1] 朱萱萱[2] 王海丹[2] 倪文澎[2] 王琼[2] 符蕊[2] 

机构地区：[1]南京中医药大学,江苏南京210029 [2]南京中医药大学附属医院,江苏南京210029

出　　处：《中华中医药学刊》2013年第5期1116-1119,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：江苏省教育厅2011年江苏省研究生创新工程项目(CXZZ11-0782);江苏省高校优势学科建设项目

摘　　要：目的:通过不同浓度、不同时间的黄芪多糖作用于人胃癌细胞系MGC-803,探讨其对胃癌细胞的生长抑制作用及细胞周期的影响。方法:用RPMI 1640培养液对人胃癌细胞MGC-803进行传代培养,采用MTT法检测黄芪多糖对MGC-803的生长抑制作用,采用流式细胞术检测黄芪多糖对MGC-803细胞周期的影响。结果:MTT法实验显示黄芪多糖可抑制MGC-803细胞增殖;细胞周期分析提示黄芪多糖可将细胞MGC-803阻滞于G0/G1期,效应均呈浓度和时间依赖性。结论:黄芪多糖能抑制胃癌细胞增殖,其机制之一是影响细胞周期使之阻滞于G0/G1期。Objective： To observe the effects of Astragalus Polysaccharide on proliferation and cell cycle of Human Gastric Carcinoma Cell Line MGC-803.Methods： Subculture was carried on by Human Gastric Carcinoma Cell Line MGC 803 in RPMI 1640 culture solution.MTT assay was used to determine the inhibitory effect of Astragalus Polysaccharide on the growth of MGG-803 cells.The changes of cell cycle of MGC-803 were detected by flow cytometry.Result： MTT assay showed that Astragalus Polysaccharide could inhibit the proliferation of cell line MGC-803,cell cycle analysis indicated that Astragalus Polysaccharide can arrest MGC-803 at G0/G1 phase,both of them were time and dose dependent manner.Conclusion： Astragalus Polysaccharides can inhibit proliferation of gastric carcinoma cells,and arrest cell cycle of G0/G1 phase,which may be one of the mechanisms.

关 键 词：黄芪多糖 胃癌细胞 细胞增殖 细胞周期 

分 类 号：R735.2[医药卫生—肿瘤]