两种不同品系小鼠的人源菌群模型的建立与肠道菌群的比较  

Establishment of two strains of HFA mice models and comparison of their intestinal microbiota

作　　者：张晓婧[1] 曾本华[2] 刘智伟[1] 廖振林[1] 方祥[1] 魏泓[2] 

机构地区：[1]华南农业大学食品学院,广东广州510642 [2]第三军医大学基础部实验动物学教研室,重庆400038

出　　处：《中国微生态学杂志》2013年第4期376-380,共5页Chinese Journal of Microecology

基　　金：国家自然科学基金(31071528);"973"计划(2013CB531406);广东省省部产学研项目(2011B090400260/2012B091100429)

摘　　要：目的比较KM(封闭群)和C57BL/6J(近交系)两种小鼠人源菌群(Human flora-associated,HFA)模型的构建过程中小鼠肠道菌群的动态变化以及定植效果。方法通过向无菌KM和C57BL/6J小鼠体内同时灌喂同一健康人的新鲜粪便,于灌喂后1、2和4周分别收集小鼠新鲜粪便。采用变性梯度凝胶电泳(Denaturing Gradient Gel Electrophoresis,DGGE)技术评价人肠道菌群在两种无菌小鼠体内的定植规律和定植效果。结果两种小鼠的菌群条带数随时间的推移而明显增加,到第4周,两种品系的小鼠的菌群的一致性均略有增高,趋于稳定。UPGMA聚类分析显示,两种小鼠分别聚在了不同的聚类枝上;并且二者在Shannon-Wiener指数上差异有统计学意义(P<0.05),在条带数S和与供体人的相似性差异有统计学意义(P<0.01),且C57BL/6J HFA小鼠模型更接近人。结论不同遗传背景的小鼠对肠道菌群具有选择性的定植效果,在多样性和与供体菌群的相似性方面,C57BL/6J HFA小鼠模型要优于KM HFA小鼠模型。Objective This study focused on the establishment of human flora-associated models of KM and C57BL/6J mice and the colonization of gut microbiota.Methods Germ-free KM and C57BL/6J mice were inoculated with fresh feces from the same person.One,two and four weeks after the inoculation,the feces of mice were collected.Denaturing Gradient Gel Electrophoresis(DGGE) was performed to evaluate the colonization.Results The DGGE profiles showed that the numbers of bands increased during the 4 weeks,and achieved consistency of certain degree respectively at last.UPGMA dendrogram demonstrated that different clades were associated with the two strains of mice;there was significant difference in Shannon-Wiener index(P<0.05) and highly significant difference in band number and similarity to the donor(P<0.01) between HFA-C57BL/6J and HFA-KM,with the former closer to the donor than the latter.Conclusion Mice with different types of genetics selectively allow for colonization of gut microbiota,and in terms of diversity and similarity to the donor,HFA-C57BL/6J was superior to HFA-KM.

关 键 词：人源菌群小鼠 无菌小鼠 KM C57BL 6J 变性梯度凝胶电泳 肠道菌群 

分 类 号：R378[医药卫生—病原生物学]