变性梯度凝胶电泳技术在微生态学研究中应用的进展  

Application of denaturing gradient gel electrophoresis technology in microecology researches:a progress review

作　　者：程翔[1] 杨景云[1] 马淑霞[1] 王春敏[1] 

机构地区：[1]佳木斯大学基础医学院微生态学教研室,黑龙江佳木斯154007

出　　处：《中国微生态学杂志》2013年第4期475-477,481,共4页Chinese Journal of Microecology

基　　金：国家"973"科学计划项目(2007CB513008)

摘　　要：变性梯度凝胶电泳(Denaturing gradient gel electrophoresis,DGGE)技术具有无需进行微生物培养、检测率高、分辨率高、重复性好等优点,现已逐渐成为微生态学研究中的强有力工具。本研究介绍了变性梯度凝胶电泳(DGGE)的基本原理和实验步骤中的系统优化,重点介绍和讨论了DGGE在微生态学研究中应用以及进展情况,并对该技术的应用前景进行了展望。Denaturing gradient gel electrophoresis(DGGE) has the advantages of no microbial culture,high detection rate,high resolution,and excellent repeatability etc.,and is now becoming a powerful tool in the study of micro-ecology.This article describes the basic principle and system optimization of experimental steps by denaturing gradient gel electrophoresis(DGGE),especially the application and progress of DGGE in microecology researches as well as the prospects of the technology.

关 键 词：变性梯度凝胶电泳 微生态学 进展 

分 类 号：TS261.1[轻工技术与工程—发酵工程]