壁虎醇提物诱导人喉癌细胞Hep2凋亡的实验研究  

Apoptosis-Inducing Activities of Gekko Ethanol Extract on Human Laryngeal Carcinoma Hep2 Cells

作　　者：崔朝初[1] 王建刚[1] 段冷昕[1] 钱昕[1] 王彩娥[2] 徐新丽[1] 

机构地区：[1]河南科技大学医学院药理学与医学分子生物学重点实验室 [2]河南科技大学第一附属医院,洛阳471003

出　　处：《天然产物研究与开发》2013年第4期551-554,共4页Natural Product Research and Development

基　　金：河南省科技重点攻关项目(102102310063);河南科技大学研究生创新基金项目(CXJJ-YJS-Z027)

摘　　要：本文研究了壁虎乙醇提取物(GEE)诱导Hep2细胞凋亡的作用。采用噻唑蓝比色法(MTT)检测了GEE对Hep2细胞增殖的抑制作用,发现GEE可呈剂量-时间依赖性的抑制Hep2细胞的增殖,GEE作用24、48、72 h后其IC50值分别为336.858、237.949、188.991μg/mL。Hoechst 33258荧光染色后在显微镜下可观察到Hep2细胞发生了典型凋亡的形态学变化;利用Western blot法证实在Hep2细胞中GEE可诱导caspase-3和抑制VEGF蛋白的表达。这些结果表明GEE可能是通过上调Caspase-3和下调VEGF蛋白的表达诱导Hep2细胞的凋亡。The current study explored the apoptosis-inducing activities of Gekko ethanol extract （GEE） on Hep2 cell line. The MTr assay showed that GEE can significantly inhibit the proliferation of Hep2 ceils in a time-dose dependent manner. After the treatment of GEE for 24 h,48 h and 72 h,the 50% inhibitory concentration （IC^o） was 336. 858, 237. 949 and 188.991 mL, respectively. The typical apoptotic morphological changes of Hep2 cells treated with GEE was observed by Hoechst 33258 staining. Furthermore, the results of western blot assay indicated that GEE can up-regulate the protein expression level of caspase-3 and suppress the expression of VEGF. In conclusion, these findings provided experimental evidences that GEE may have apoptosis-inducing effects on Hep2 cells by increasing protein expression of caspase-3 and down-regulating VEGF.

关 键 词：壁虎乙醇提取物 Hep2细胞 凋亡 CASPASE 3 VEGF 

分 类 号：R285.5[医药卫生—中药学]